Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera β-glucosidase

Eugene Jeon, Jeong Eun Hyeon, Lee Sung Eun, Byeoung Soo Park, Seung Wook Kim, Jinwon Lee, Sung Ok Han

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In this study, Saccharomyces cerevisiae was engineered for simultaneous saccharification and fermentation of cellulose by the overexpression of the endoglucanase D (EngD) from Clostridium cellulovorans and the β-glucosidase (Bgl1) from Saccharomycopsis fibuligera. To promote secretion of the two enzymes, the genes were fused to the secretion signal of the S. cerevisiae α mating factor gene. The recombinant developed yeast could produce ethanol through simultaneous production of sufficient extracellular endoglucanase and β-glucosidase. When direct ethanol fermentation from 20 g L-1 β-glucan as a substrate was performed with our recombinant strains, the ethanol concentration reached 9.15 g L-1 after 50 h of fermentation. The conversion ratio of ethanol from β-glucan was 80.3% of the theoretical ethanol concentration produced from 20 g L-1 β-glucan. In conclusion, we have demonstrated the construction of a yeast strain capable of conversion of a cellulosic substrate to ethanol, representing significant progress towards the realization of processing of cellulosic biomass in a consolidated bioprocessing configuration.

Original languageEnglish
Pages (from-to)130-136
Number of pages7
JournalFEMS Microbiology Letters
Volume301
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Clostridium cellulovorans
Saccharomycopsis
Glucosidases
Cellulase
Fermentation
Saccharomyces cerevisiae
Ethanol
Glucans
Mating Factor
Yeasts
Cellulose
Biomass
Genes

Keywords

  • β-glucosidase
  • Cellulose degradation
  • Clostridium cellulovorans
  • Ethanol production
  • Noncellulosomal endoglucanase
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera β-glucosidase. / Jeon, Eugene; Hyeon, Jeong Eun; Sung Eun, Lee; Park, Byeoung Soo; Kim, Seung Wook; Lee, Jinwon; Han, Sung Ok.

In: FEMS Microbiology Letters, Vol. 301, No. 1, 01.01.2009, p. 130-136.

Research output: Contribution to journalArticle

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