Abstract
The cellulosome is one of nature's most elegant and elaborate nanomachines and a key biological and biotechnological macromolecule that can be used as a multi-functional protein complex tool. Each protein module in the cellulosome system is potentially useful in an advanced biotechnology application. The high-affinity interactions between the cohesin and dockerin domains can be used in protein-based biosensors to improve both sensitivity and selectivity. The scaffolding protein includes a carbohydrate-binding module (CBM) that attaches strongly to cellulose substrates and facilitates the purification of proteins fused with the dockerin module through a one-step CBM purification method. Although the surface layer homology (SLH) domain of CbpA is not present in other strains, replacement of the cell surface anchoring domain allows a foreign protein to be displayed on the surface of other strains. The development of a hydrolysis enzyme complex is a useful strategy for consolidated bioprocessing (CBP), enabling microorganisms with biomass hydrolysis activity. Thus, the development of various configurations of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications. This review provides a detailed summary of the current achievements in Clostridium-derived multi-functional complex development and the impact of these complexes in various areas of biotechnology.
| Original language | English |
|---|---|
| Pages (from-to) | 936-944 |
| Number of pages | 9 |
| Journal | Biotechnology Advances |
| Volume | 31 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2013 Nov 1 |
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Keywords
- Biosensor
- Carbohydrate binding module
- Cell surface anchoring
- Cellulosome
- Clostridium cellulovorans
- Cohesin-dockerin interaction
- Consolidated bioprocessing
- Designer minicellulosome
- One-step CBM purification
- Whole-cell biocatalyst
ASJC Scopus subject areas
- Biotechnology
Cite this
Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development : Advances and applications. / Hyeon, Jeong Eun; Jeon, Sang Duck; Han, Sung Ok.
In: Biotechnology Advances, Vol. 31, No. 6, 01.11.2013, p. 936-944.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development
T2 - Advances and applications
AU - Hyeon, Jeong Eun
AU - Jeon, Sang Duck
AU - Han, Sung Ok
PY - 2013/11/1
Y1 - 2013/11/1
N2 - The cellulosome is one of nature's most elegant and elaborate nanomachines and a key biological and biotechnological macromolecule that can be used as a multi-functional protein complex tool. Each protein module in the cellulosome system is potentially useful in an advanced biotechnology application. The high-affinity interactions between the cohesin and dockerin domains can be used in protein-based biosensors to improve both sensitivity and selectivity. The scaffolding protein includes a carbohydrate-binding module (CBM) that attaches strongly to cellulose substrates and facilitates the purification of proteins fused with the dockerin module through a one-step CBM purification method. Although the surface layer homology (SLH) domain of CbpA is not present in other strains, replacement of the cell surface anchoring domain allows a foreign protein to be displayed on the surface of other strains. The development of a hydrolysis enzyme complex is a useful strategy for consolidated bioprocessing (CBP), enabling microorganisms with biomass hydrolysis activity. Thus, the development of various configurations of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications. This review provides a detailed summary of the current achievements in Clostridium-derived multi-functional complex development and the impact of these complexes in various areas of biotechnology.
AB - The cellulosome is one of nature's most elegant and elaborate nanomachines and a key biological and biotechnological macromolecule that can be used as a multi-functional protein complex tool. Each protein module in the cellulosome system is potentially useful in an advanced biotechnology application. The high-affinity interactions between the cohesin and dockerin domains can be used in protein-based biosensors to improve both sensitivity and selectivity. The scaffolding protein includes a carbohydrate-binding module (CBM) that attaches strongly to cellulose substrates and facilitates the purification of proteins fused with the dockerin module through a one-step CBM purification method. Although the surface layer homology (SLH) domain of CbpA is not present in other strains, replacement of the cell surface anchoring domain allows a foreign protein to be displayed on the surface of other strains. The development of a hydrolysis enzyme complex is a useful strategy for consolidated bioprocessing (CBP), enabling microorganisms with biomass hydrolysis activity. Thus, the development of various configurations of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications. This review provides a detailed summary of the current achievements in Clostridium-derived multi-functional complex development and the impact of these complexes in various areas of biotechnology.
KW - Biosensor
KW - Carbohydrate binding module
KW - Cell surface anchoring
KW - Cellulosome
KW - Clostridium cellulovorans
KW - Cohesin-dockerin interaction
KW - Consolidated bioprocessing
KW - Designer minicellulosome
KW - One-step CBM purification
KW - Whole-cell biocatalyst
UR - http://www.scopus.com/inward/record.url?scp=84882662469&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882662469&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2013.03.009
DO - 10.1016/j.biotechadv.2013.03.009
M3 - Article
C2 - 23563098
AN - SCOPUS:84882662469
VL - 31
SP - 936
EP - 944
JO - Biotechnology Advances
JF - Biotechnology Advances
SN - 0734-9750
IS - 6
ER -