TY - JOUR
T1 - Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development
T2 - Advances and applications
AU - Hyeon, Jeong Eun
AU - Jeon, Sang Duck
AU - Han, Sung Ok
N1 - Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012R1A1A2005630 ).
PY - 2013/11/1
Y1 - 2013/11/1
N2 - The cellulosome is one of nature's most elegant and elaborate nanomachines and a key biological and biotechnological macromolecule that can be used as a multi-functional protein complex tool. Each protein module in the cellulosome system is potentially useful in an advanced biotechnology application. The high-affinity interactions between the cohesin and dockerin domains can be used in protein-based biosensors to improve both sensitivity and selectivity. The scaffolding protein includes a carbohydrate-binding module (CBM) that attaches strongly to cellulose substrates and facilitates the purification of proteins fused with the dockerin module through a one-step CBM purification method. Although the surface layer homology (SLH) domain of CbpA is not present in other strains, replacement of the cell surface anchoring domain allows a foreign protein to be displayed on the surface of other strains. The development of a hydrolysis enzyme complex is a useful strategy for consolidated bioprocessing (CBP), enabling microorganisms with biomass hydrolysis activity. Thus, the development of various configurations of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications. This review provides a detailed summary of the current achievements in Clostridium-derived multi-functional complex development and the impact of these complexes in various areas of biotechnology.
AB - The cellulosome is one of nature's most elegant and elaborate nanomachines and a key biological and biotechnological macromolecule that can be used as a multi-functional protein complex tool. Each protein module in the cellulosome system is potentially useful in an advanced biotechnology application. The high-affinity interactions between the cohesin and dockerin domains can be used in protein-based biosensors to improve both sensitivity and selectivity. The scaffolding protein includes a carbohydrate-binding module (CBM) that attaches strongly to cellulose substrates and facilitates the purification of proteins fused with the dockerin module through a one-step CBM purification method. Although the surface layer homology (SLH) domain of CbpA is not present in other strains, replacement of the cell surface anchoring domain allows a foreign protein to be displayed on the surface of other strains. The development of a hydrolysis enzyme complex is a useful strategy for consolidated bioprocessing (CBP), enabling microorganisms with biomass hydrolysis activity. Thus, the development of various configurations of multi-functional protein complexes for use as tools in whole-cell biocatalyst systems has drawn considerable attention as an attractive strategy for bioprocess applications. This review provides a detailed summary of the current achievements in Clostridium-derived multi-functional complex development and the impact of these complexes in various areas of biotechnology.
KW - Biosensor
KW - Carbohydrate binding module
KW - Cell surface anchoring
KW - Cellulosome
KW - Clostridium cellulovorans
KW - Cohesin-dockerin interaction
KW - Consolidated bioprocessing
KW - Designer minicellulosome
KW - One-step CBM purification
KW - Whole-cell biocatalyst
UR - http://www.scopus.com/inward/record.url?scp=84882662469&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2013.03.009
DO - 10.1016/j.biotechadv.2013.03.009
M3 - Review article
C2 - 23563098
AN - SCOPUS:84882662469
VL - 31
SP - 936
EP - 944
JO - Biotechnology Advances
JF - Biotechnology Advances
SN - 0734-9750
IS - 6
ER -