Cephalexin synthesis using immobilized Xanthomonas citri cells

Ik H. Kim, D. H. Nam, Dewey D.Y. Ryu

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

For continuous production of cephalexin, whole cells of Xanthomonas citri were immobilized by entrapment in polyacrylamide gel and kappa-carrageenan gel. It wasfound that cells immobilized with kappa-carrageenan showed better thermal stability compared to those immobilized by polyacrylamide gel. The cells immobilized with kappa-carrageenan were treated with glutaraldehyde and hexamethylenediamine to prevent gel destruction during prolonged operation. By immobilizing intact cells, the optimal temperature for the synthetic enzyme reaction shifted higher by 8°C and the optimal pH became broader around 6.2 In continuous operation, the immobilized cells retained better operational stability at 25 than at 37°C, and also showed maximal conversion up to 83% at 25°C.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume8
Issue number3
DOIs
Publication statusPublished - 1983 Jun
Externally publishedYes

Keywords

  • Cephalexin, synthesis using immobilized cells
  • Xanthomonas citri cells, cephalexin synthesis using immobilized
  • immobilized cells, in cephalexin synthesis
  • k-carrageenan immobilization, of Xanthomonas citri
  • polyacrylamide gel immobilization, of Xanthamonas citri cells
  • synthesis, of cephalexin using immobilized cells

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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