Changes in awake cystometry and expression of bladder β-adrenoceptors after partial bladder outlet obstruction in male rats

Min Gu Park, Hong Seok Park, Jeong Gu Lee, Hyung Jee Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: To explore possible changes in awake cystometry and expression of beta-adrenoceptors (ARs) as a cause for bladder dysfunction in a male rat model of partial bladder outlet obstruction (pBOO). Materials and Methods: Awake cystometry was performed in rats with pBOO (16) and sham-operated rats (16), 8 weeks after the operation. The expression of mRNA and protein of β-ARs was assessed by real-time PCR and western blot. Results: The bladders with pBOO (1030mg) were increased compared to those in control rats (230mg). In the cystometric studies, the maximum intravesical pressure significantly increased in the pBOO group compared to control group rats (p=0.001). The time to reach maximal intravesical pressure during micturition in the pBOO group was significantly longer than the sham group (p=0.003). The frequency of non-void contraction in the pBOO group was significantly more than the sham group (p=0.006). The mRNA expressions of β2- and 3-ARs were increased insignificantly in pBOO group compared to sham group. The data of pBOO group expressed as folds of corresponding expression in sham group were 1.28 and 1.46 respectively in β2- and 3-ARs. Compared to the sham groups, the density of 60Kda protein band recognized by β2-AR antibodies and the density of 45Kda protein band recognized by β3-AR antibodies were higher in the bladder from pBOO group rats. Conclusion: PBOO of male rats increase the maximal intravesical pressure and contraction time during micturition and the frequency of non-void contraction as well as weight of bladder. The expression of β2-and 3-ARs subtypes was increased insignificantly compared to sham operated group. This study demonstrates that the changes of cystometric or non-void contraction parameters in pBOO is one of the pathophysiologic processes potentially associated with the alterations of bladder β-ARs.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalInternational Neurourology Journal
Volume14
Issue number3
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

Urinary Bladder Neck Obstruction
Adrenergic Receptors
Urinary Bladder
Urination
Pressure
Talin
Erythrocyte Anion Exchange Protein 1
Messenger RNA
Antibodies
Real-Time Polymerase Chain Reaction
Western Blotting

Keywords

  • Beta-adrenoceptor
  • Bladder
  • Bladder outlet obstruction
  • Rat

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Urology

Cite this

Changes in awake cystometry and expression of bladder β-adrenoceptors after partial bladder outlet obstruction in male rats. / Park, Min Gu; Park, Hong Seok; Lee, Jeong Gu; Kim, Hyung Jee.

In: International Neurourology Journal, Vol. 14, No. 3, 01.10.2010, p. 157-163.

Research output: Contribution to journalArticle

@article{1cb24d72c9b6496cbf96d08131e5a9ee,
title = "Changes in awake cystometry and expression of bladder β-adrenoceptors after partial bladder outlet obstruction in male rats",
abstract = "Purpose: To explore possible changes in awake cystometry and expression of beta-adrenoceptors (ARs) as a cause for bladder dysfunction in a male rat model of partial bladder outlet obstruction (pBOO). Materials and Methods: Awake cystometry was performed in rats with pBOO (16) and sham-operated rats (16), 8 weeks after the operation. The expression of mRNA and protein of β-ARs was assessed by real-time PCR and western blot. Results: The bladders with pBOO (1030mg) were increased compared to those in control rats (230mg). In the cystometric studies, the maximum intravesical pressure significantly increased in the pBOO group compared to control group rats (p=0.001). The time to reach maximal intravesical pressure during micturition in the pBOO group was significantly longer than the sham group (p=0.003). The frequency of non-void contraction in the pBOO group was significantly more than the sham group (p=0.006). The mRNA expressions of β2- and 3-ARs were increased insignificantly in pBOO group compared to sham group. The data of pBOO group expressed as folds of corresponding expression in sham group were 1.28 and 1.46 respectively in β2- and 3-ARs. Compared to the sham groups, the density of 60Kda protein band recognized by β2-AR antibodies and the density of 45Kda protein band recognized by β3-AR antibodies were higher in the bladder from pBOO group rats. Conclusion: PBOO of male rats increase the maximal intravesical pressure and contraction time during micturition and the frequency of non-void contraction as well as weight of bladder. The expression of β2-and 3-ARs subtypes was increased insignificantly compared to sham operated group. This study demonstrates that the changes of cystometric or non-void contraction parameters in pBOO is one of the pathophysiologic processes potentially associated with the alterations of bladder β-ARs.",
keywords = "Beta-adrenoceptor, Bladder, Bladder outlet obstruction, Rat",
author = "Park, {Min Gu} and Park, {Hong Seok} and Lee, {Jeong Gu} and Kim, {Hyung Jee}",
year = "2010",
month = "10",
day = "1",
doi = "10.5213/inj.2010.14.3.157",
language = "English",
volume = "14",
pages = "157--163",
journal = "International Neurourology Journal",
issn = "2093-4777",
publisher = "Korean Association of Medical Journal Edirors",
number = "3",

}

TY - JOUR

T1 - Changes in awake cystometry and expression of bladder β-adrenoceptors after partial bladder outlet obstruction in male rats

AU - Park, Min Gu

AU - Park, Hong Seok

AU - Lee, Jeong Gu

AU - Kim, Hyung Jee

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Purpose: To explore possible changes in awake cystometry and expression of beta-adrenoceptors (ARs) as a cause for bladder dysfunction in a male rat model of partial bladder outlet obstruction (pBOO). Materials and Methods: Awake cystometry was performed in rats with pBOO (16) and sham-operated rats (16), 8 weeks after the operation. The expression of mRNA and protein of β-ARs was assessed by real-time PCR and western blot. Results: The bladders with pBOO (1030mg) were increased compared to those in control rats (230mg). In the cystometric studies, the maximum intravesical pressure significantly increased in the pBOO group compared to control group rats (p=0.001). The time to reach maximal intravesical pressure during micturition in the pBOO group was significantly longer than the sham group (p=0.003). The frequency of non-void contraction in the pBOO group was significantly more than the sham group (p=0.006). The mRNA expressions of β2- and 3-ARs were increased insignificantly in pBOO group compared to sham group. The data of pBOO group expressed as folds of corresponding expression in sham group were 1.28 and 1.46 respectively in β2- and 3-ARs. Compared to the sham groups, the density of 60Kda protein band recognized by β2-AR antibodies and the density of 45Kda protein band recognized by β3-AR antibodies were higher in the bladder from pBOO group rats. Conclusion: PBOO of male rats increase the maximal intravesical pressure and contraction time during micturition and the frequency of non-void contraction as well as weight of bladder. The expression of β2-and 3-ARs subtypes was increased insignificantly compared to sham operated group. This study demonstrates that the changes of cystometric or non-void contraction parameters in pBOO is one of the pathophysiologic processes potentially associated with the alterations of bladder β-ARs.

AB - Purpose: To explore possible changes in awake cystometry and expression of beta-adrenoceptors (ARs) as a cause for bladder dysfunction in a male rat model of partial bladder outlet obstruction (pBOO). Materials and Methods: Awake cystometry was performed in rats with pBOO (16) and sham-operated rats (16), 8 weeks after the operation. The expression of mRNA and protein of β-ARs was assessed by real-time PCR and western blot. Results: The bladders with pBOO (1030mg) were increased compared to those in control rats (230mg). In the cystometric studies, the maximum intravesical pressure significantly increased in the pBOO group compared to control group rats (p=0.001). The time to reach maximal intravesical pressure during micturition in the pBOO group was significantly longer than the sham group (p=0.003). The frequency of non-void contraction in the pBOO group was significantly more than the sham group (p=0.006). The mRNA expressions of β2- and 3-ARs were increased insignificantly in pBOO group compared to sham group. The data of pBOO group expressed as folds of corresponding expression in sham group were 1.28 and 1.46 respectively in β2- and 3-ARs. Compared to the sham groups, the density of 60Kda protein band recognized by β2-AR antibodies and the density of 45Kda protein band recognized by β3-AR antibodies were higher in the bladder from pBOO group rats. Conclusion: PBOO of male rats increase the maximal intravesical pressure and contraction time during micturition and the frequency of non-void contraction as well as weight of bladder. The expression of β2-and 3-ARs subtypes was increased insignificantly compared to sham operated group. This study demonstrates that the changes of cystometric or non-void contraction parameters in pBOO is one of the pathophysiologic processes potentially associated with the alterations of bladder β-ARs.

KW - Beta-adrenoceptor

KW - Bladder

KW - Bladder outlet obstruction

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=84859496203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859496203&partnerID=8YFLogxK

U2 - 10.5213/inj.2010.14.3.157

DO - 10.5213/inj.2010.14.3.157

M3 - Article

VL - 14

SP - 157

EP - 163

JO - International Neurourology Journal

JF - International Neurourology Journal

SN - 2093-4777

IS - 3

ER -