Changes in cell size and buoyant density of Pseudomonas diminuta in response to osmotic shocks

So Hee Lee, Yu Ree Cho, Yong Jin Choi, Chan Wha Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pseudomonas diminuta (ATCC 19146) has been typically used in the bacterial challenge test for validation of the sterilizing filtration process. Cell size is critical for determining the retention characteristics of membrane filters with pore-size of 0.2 μm. The changes of cell sizes after osmotic shocks at 150, 260, 500, and 700 mosM were measured by a particle size analyzer and the changes of their buoyant densities were analyzed with a Percoll gradient. The results indicated that there were no significant differences when cells were cultured in 260 mosM medium and osmotically shocked at 500 and 700 mosM. However, the osmotically shocked cells at 150 mosM showed a 38% increase of the cell size compared to the cells at 260 mosM. From these study, we concluded that the worst case condition for validation of a sterilizing filter would be 500 mosM, not because of changes in the cell size, but due to decrease in cell viability under those conditions.

Original languageEnglish
Pages (from-to)326-328
Number of pages3
JournalJournal of Microbiology and Biotechnology
Volume11
Issue number2
Publication statusPublished - 2001 Apr 1

Fingerprint

Osmotic Pressure
Pseudomonas
Cell Size
Pore size
Particle size
Cells
Membranes
Particle Size
Cultured Cells
Cell Survival
Percoll

Keywords

  • Buoyant density
  • Filter validation
  • Osmolarity
  • Pseudomonas diminuta

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Changes in cell size and buoyant density of Pseudomonas diminuta in response to osmotic shocks. / Lee, So Hee; Cho, Yu Ree; Choi, Yong Jin; Kim, Chan Wha.

In: Journal of Microbiology and Biotechnology, Vol. 11, No. 2, 01.04.2001, p. 326-328.

Research output: Contribution to journalArticle

@article{6c22d8ab21374710907020ef1862fc5e,
title = "Changes in cell size and buoyant density of Pseudomonas diminuta in response to osmotic shocks",
abstract = "Pseudomonas diminuta (ATCC 19146) has been typically used in the bacterial challenge test for validation of the sterilizing filtration process. Cell size is critical for determining the retention characteristics of membrane filters with pore-size of 0.2 μm. The changes of cell sizes after osmotic shocks at 150, 260, 500, and 700 mosM were measured by a particle size analyzer and the changes of their buoyant densities were analyzed with a Percoll gradient. The results indicated that there were no significant differences when cells were cultured in 260 mosM medium and osmotically shocked at 500 and 700 mosM. However, the osmotically shocked cells at 150 mosM showed a 38{\%} increase of the cell size compared to the cells at 260 mosM. From these study, we concluded that the worst case condition for validation of a sterilizing filter would be 500 mosM, not because of changes in the cell size, but due to decrease in cell viability under those conditions.",
keywords = "Buoyant density, Filter validation, Osmolarity, Pseudomonas diminuta",
author = "Lee, {So Hee} and Cho, {Yu Ree} and Choi, {Yong Jin} and Kim, {Chan Wha}",
year = "2001",
month = "4",
day = "1",
language = "English",
volume = "11",
pages = "326--328",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "2",

}

TY - JOUR

T1 - Changes in cell size and buoyant density of Pseudomonas diminuta in response to osmotic shocks

AU - Lee, So Hee

AU - Cho, Yu Ree

AU - Choi, Yong Jin

AU - Kim, Chan Wha

PY - 2001/4/1

Y1 - 2001/4/1

N2 - Pseudomonas diminuta (ATCC 19146) has been typically used in the bacterial challenge test for validation of the sterilizing filtration process. Cell size is critical for determining the retention characteristics of membrane filters with pore-size of 0.2 μm. The changes of cell sizes after osmotic shocks at 150, 260, 500, and 700 mosM were measured by a particle size analyzer and the changes of their buoyant densities were analyzed with a Percoll gradient. The results indicated that there were no significant differences when cells were cultured in 260 mosM medium and osmotically shocked at 500 and 700 mosM. However, the osmotically shocked cells at 150 mosM showed a 38% increase of the cell size compared to the cells at 260 mosM. From these study, we concluded that the worst case condition for validation of a sterilizing filter would be 500 mosM, not because of changes in the cell size, but due to decrease in cell viability under those conditions.

AB - Pseudomonas diminuta (ATCC 19146) has been typically used in the bacterial challenge test for validation of the sterilizing filtration process. Cell size is critical for determining the retention characteristics of membrane filters with pore-size of 0.2 μm. The changes of cell sizes after osmotic shocks at 150, 260, 500, and 700 mosM were measured by a particle size analyzer and the changes of their buoyant densities were analyzed with a Percoll gradient. The results indicated that there were no significant differences when cells were cultured in 260 mosM medium and osmotically shocked at 500 and 700 mosM. However, the osmotically shocked cells at 150 mosM showed a 38% increase of the cell size compared to the cells at 260 mosM. From these study, we concluded that the worst case condition for validation of a sterilizing filter would be 500 mosM, not because of changes in the cell size, but due to decrease in cell viability under those conditions.

KW - Buoyant density

KW - Filter validation

KW - Osmolarity

KW - Pseudomonas diminuta

UR - http://www.scopus.com/inward/record.url?scp=6644228072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6644228072&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 326

EP - 328

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 2

ER -