Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins

Jae Sang Hong, Nam Hoon Kim, Chang Yong Choi, Jun Seong Lee, Dokyun Na, Taehoon Chun, Youngsik Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Porcine circovirus type 2 (PCV2) is the primary causative agent of postweaning multisystemic wasting syndrome, which leads to serious economic losses in the pig industry worldwide. While the molecular basis of PCV2 replication and pathogenicity remains elusive, it is increasingly apparent that the microRNA (miRNA) pathway plays a key role in controlling virus-host interactions, in addition to a wide range of cellular processes. Here, we employed Solexa deep sequencing technology to determine which cellular miRNAs were differentially regulated after expression of each of three PCV2-encoded open reading frames (ORFs) in porcine kidney epithelial (PK15) cells. We identified 51 ORF1-regulated miRNAs, 74 ORF2-regulated miRNAs, and 32 ORF3-regulated miRNAs that differed in abundance compared to the control. Gene ontology analysis of the putative targets of these miRNAs identified transcriptional regulation as the most significantly enriched biological process, while KEGG pathway analysis revealed significant enrichment for several pathways including MAPK signaling, which is activated during PCV2 infection. Among the potential target genes of ORF-regulated miRNAs, two genes encoding proteins that are known to interact with PCV2-encoded proteins, zinc finger protein 265 (ZNF265) and regulator of G protein signaling 16 (RGS16), were selected for further analysis. We provide evidence that ZNF265 and RGS16 are direct targets of miR-139-5p and let-7e, respectively, which are both down-regulated by ORF2. Our data will initiate further studies to elucidate the roles of ORF-regulated cellular miRNAs in PCV2-host interactions.

Original languageEnglish
Article number39
JournalVeterinary Research
Volume46
Issue number1
DOIs
Publication statusPublished - 2015 Apr 10

Fingerprint

Circovirus
Porcine circovirus-2
MicroRNAs
microRNA
open reading frames
Proteins
proteins
zinc finger motif
Open Reading Frames
G-proteins
Zinc Fingers
postweaning multisystemic wasting syndrome
Circoviridae Infections
swine
Swine
genes
kidney cells
Wasting Syndrome
Biological Phenomena
High-Throughput Nucleotide Sequencing

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins. / Hong, Jae Sang; Kim, Nam Hoon; Choi, Chang Yong; Lee, Jun Seong; Na, Dokyun; Chun, Taehoon; Lee, Youngsik.

In: Veterinary Research, Vol. 46, No. 1, 39, 10.04.2015.

Research output: Contribution to journalArticle

Hong, Jae Sang ; Kim, Nam Hoon ; Choi, Chang Yong ; Lee, Jun Seong ; Na, Dokyun ; Chun, Taehoon ; Lee, Youngsik. / Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins. In: Veterinary Research. 2015 ; Vol. 46, No. 1.
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