Changes in locomotor activity and oxidative stress-related factors after the administration of an amino acid mixture by generation and age

Yejin Ahn, Ki Bae Hong, Suhyeon Kim, Hyung Joo Suh, Kyungae Jo

Research output: Contribution to journalArticlepeer-review

Abstract

Amino acids, as nutrients, are expected to improve sleep disorders. This study aimed to evaluate the generation-and age-dependent sleep-improving effects of γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) coadministration. The differentially expressed genes and generation-related behavior after the administration of a GABA/5-HTP mixture were measured in a Drosophila model, while age-related changes in gene expression and oxidative stress-related parameters were measured in a mouse model. The GABA/5-HTP-treated group showed significant behavioral changes compared to the other groups. Sequencing revealed that the GABA/5-HTP mixture influenced changes in nervous system-related genes, including those involved in the regulation of the expression of behavioral and synaptic genes. Additionally, total sleep time increased with age, and nighttime sleep time in the first-and third-generation flies was significantly different from that of the control groups. The GABA/5-HTP mixture induced significant changes in the expression of sleep-related receptors in both models. Furthermore, the GABA/5-HTP mixture reduced levels of ROS and ROS reaction products in an age-dependent manner. Therefore, the increase in behavioral changes caused by GABA/5-HTP mixture administration was effective in eliminating ROS activity across generations and ages.

Original languageEnglish
Article number9822
JournalInternational journal of molecular sciences
Volume22
Issue number18
DOIs
Publication statusPublished - 2021 Sep

Keywords

  • 5-hydroxytryptophan
  • Age
  • Aminobutyric acid
  • Generation
  • Reactive oxygen species
  • Sleep

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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