Changes in nerve growth factor levels in dorsal root ganglia and spinal nerves in a rat neuropathic pain model

Eun Joo Oh, Young Wook Yoon, Seo Eun Lee, Seung Kil Hong

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The purpose of this study was to measure the changes in levels of nerve growth factor (NGF) in dorsal root ganglia (DRG) and spinal nerves with the aim of investigating the role of NGF in a rat neuropathic pain model. Nerve injuries were made by tight ligation of the left L5 and L6 spinal nerves using 6-0 silk thread in male Sprague-Dawley rats. Before surgery and 1, 3, 5, 7, and 14 days after surgery, tissue samples collected included the L3-6 DRGs bilaterally, segments of the ipsilateral L5-6 spinal nerves proximal and distal to ligation sites, and corresponding sites of the contralateral L3-6 and the ipsilateral L3-4 spinal nerves. NGF levels in the DRGs of the injured spinal nerves (the left L5 and L6) did not change significantly from control values. The spinal nerve segments distal to ligation sites had higher levels of NGF than the control values. Unlesioned sites did not show any significant changes in NGF levels. The increase of NGF in distal segments of injured spinal nerves may be due to an accumulation of retrogradely transported NGF. The maintenance of NGF levels in the DRGs that had lost peripheral connections may reflect local synthesis after nerve injury.

Original languageEnglish
Pages (from-to)93-99
Number of pages7
JournalExperimental Brain Research
Volume130
Issue number1
Publication statusPublished - 2000 Feb 23

Fingerprint

Spinal Nerves
Spinal Ganglia
Neuralgia
Nerve Growth Factor
Diagnosis-Related Groups
Ligation
Silk
Wounds and Injuries
Ambulatory Surgical Procedures
Sprague Dawley Rats
Maintenance

Keywords

  • Dorsal root ganglia
  • Mechanical allodynia
  • Nerve growth factor (NGF)
  • Neuropathic pain
  • Peripheral nerve injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Changes in nerve growth factor levels in dorsal root ganglia and spinal nerves in a rat neuropathic pain model. / Oh, Eun Joo; Yoon, Young Wook; Lee, Seo Eun; Hong, Seung Kil.

In: Experimental Brain Research, Vol. 130, No. 1, 23.02.2000, p. 93-99.

Research output: Contribution to journalArticle

@article{021353836af940188c9c818b51c52b49,
title = "Changes in nerve growth factor levels in dorsal root ganglia and spinal nerves in a rat neuropathic pain model",
abstract = "The purpose of this study was to measure the changes in levels of nerve growth factor (NGF) in dorsal root ganglia (DRG) and spinal nerves with the aim of investigating the role of NGF in a rat neuropathic pain model. Nerve injuries were made by tight ligation of the left L5 and L6 spinal nerves using 6-0 silk thread in male Sprague-Dawley rats. Before surgery and 1, 3, 5, 7, and 14 days after surgery, tissue samples collected included the L3-6 DRGs bilaterally, segments of the ipsilateral L5-6 spinal nerves proximal and distal to ligation sites, and corresponding sites of the contralateral L3-6 and the ipsilateral L3-4 spinal nerves. NGF levels in the DRGs of the injured spinal nerves (the left L5 and L6) did not change significantly from control values. The spinal nerve segments distal to ligation sites had higher levels of NGF than the control values. Unlesioned sites did not show any significant changes in NGF levels. The increase of NGF in distal segments of injured spinal nerves may be due to an accumulation of retrogradely transported NGF. The maintenance of NGF levels in the DRGs that had lost peripheral connections may reflect local synthesis after nerve injury.",
keywords = "Dorsal root ganglia, Mechanical allodynia, Nerve growth factor (NGF), Neuropathic pain, Peripheral nerve injury",
author = "Oh, {Eun Joo} and Yoon, {Young Wook} and Lee, {Seo Eun} and Hong, {Seung Kil}",
year = "2000",
month = "2",
day = "23",
language = "English",
volume = "130",
pages = "93--99",
journal = "Experimental Brain Research",
issn = "0014-4819",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Changes in nerve growth factor levels in dorsal root ganglia and spinal nerves in a rat neuropathic pain model

AU - Oh, Eun Joo

AU - Yoon, Young Wook

AU - Lee, Seo Eun

AU - Hong, Seung Kil

PY - 2000/2/23

Y1 - 2000/2/23

N2 - The purpose of this study was to measure the changes in levels of nerve growth factor (NGF) in dorsal root ganglia (DRG) and spinal nerves with the aim of investigating the role of NGF in a rat neuropathic pain model. Nerve injuries were made by tight ligation of the left L5 and L6 spinal nerves using 6-0 silk thread in male Sprague-Dawley rats. Before surgery and 1, 3, 5, 7, and 14 days after surgery, tissue samples collected included the L3-6 DRGs bilaterally, segments of the ipsilateral L5-6 spinal nerves proximal and distal to ligation sites, and corresponding sites of the contralateral L3-6 and the ipsilateral L3-4 spinal nerves. NGF levels in the DRGs of the injured spinal nerves (the left L5 and L6) did not change significantly from control values. The spinal nerve segments distal to ligation sites had higher levels of NGF than the control values. Unlesioned sites did not show any significant changes in NGF levels. The increase of NGF in distal segments of injured spinal nerves may be due to an accumulation of retrogradely transported NGF. The maintenance of NGF levels in the DRGs that had lost peripheral connections may reflect local synthesis after nerve injury.

AB - The purpose of this study was to measure the changes in levels of nerve growth factor (NGF) in dorsal root ganglia (DRG) and spinal nerves with the aim of investigating the role of NGF in a rat neuropathic pain model. Nerve injuries were made by tight ligation of the left L5 and L6 spinal nerves using 6-0 silk thread in male Sprague-Dawley rats. Before surgery and 1, 3, 5, 7, and 14 days after surgery, tissue samples collected included the L3-6 DRGs bilaterally, segments of the ipsilateral L5-6 spinal nerves proximal and distal to ligation sites, and corresponding sites of the contralateral L3-6 and the ipsilateral L3-4 spinal nerves. NGF levels in the DRGs of the injured spinal nerves (the left L5 and L6) did not change significantly from control values. The spinal nerve segments distal to ligation sites had higher levels of NGF than the control values. Unlesioned sites did not show any significant changes in NGF levels. The increase of NGF in distal segments of injured spinal nerves may be due to an accumulation of retrogradely transported NGF. The maintenance of NGF levels in the DRGs that had lost peripheral connections may reflect local synthesis after nerve injury.

KW - Dorsal root ganglia

KW - Mechanical allodynia

KW - Nerve growth factor (NGF)

KW - Neuropathic pain

KW - Peripheral nerve injury

UR - http://www.scopus.com/inward/record.url?scp=0033950111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033950111&partnerID=8YFLogxK

M3 - Article

C2 - 10638445

AN - SCOPUS:0033950111

VL - 130

SP - 93

EP - 99

JO - Experimental Brain Research

JF - Experimental Brain Research

SN - 0014-4819

IS - 1

ER -