Changes in RBC deformability and oxygen-delivering ability in cold blood cardioplegia

Kuk Hui Son, Chi Bum Ahn, Sung Ho Lee, Insup Noh, Eunkyoung Shin, Mijin So, Hyuna Kim, Kyung Sun, Kwang Taik Kim

Research output: Contribution to journalArticle

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Abstract

Purpose: The advantages of blood cardioplegia, which is used for myocardial protection during open heart surgeries, include superior oxygen-carrying capacities, better osmotic properties, and the presence of more antioxidants than a crystalloid counterpart. Although, hyperkalemic organ-preserving solutions for transplantation surgeries are known to decrease RBC deformability essential for tissue perfusion, only few studies have addressed the changes in RBC deformability after exposure to cardioplegic additives. The purpose of this study was to measure deformability and oxygen-delivery capacities in various blood cardioplegic solutions. Methods: Blood from eight healthy volunteers was used. Each sample (100 ml) was divided into 5 groups of 16 ml, and cardioplegia solutions were added (group NS; blood + normal saline, group K; blood + KCl, group D; blood + KCl + diltiazem, group A: blood + KCl + adenosine, group E: blood + KCl + neutrophil elastase inhibitor [Sivelestat]). All samples were incubated at a temperature of 8°C for 10 minutes. Deformability, NO level, 2,3-DPG, and ATP were measured. Results: There was no statistically significant difference (p = 0.92) in deformability between the groups. The NO levels were not significantly different (p = 0.86). The 2,3-DPG (p = 0.27) and ATP levels (p = 0.40) were not significantly different. Conclusions: The deformability and oxygen carrying functions of RBCs did not show a significant difference according to various components of cold blood cardioplegia during 10 minutes of incubation.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalClinical Hemorheology and Microcirculation
Volume48
Issue number4
DOIs
Publication statusPublished - 2011 Nov 7

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Induced Heart Arrest
Oxygen
Blood Group Antigens
2,3-Diphosphoglycerate
Secretory Proteinase Inhibitory Proteins
Adenosine Triphosphate
Cardioplegic Solutions
Diltiazem
Conservation of Natural Resources
Adenosine
Thoracic Surgery
Healthy Volunteers
Perfusion
Antioxidants
Transplantation
Temperature

Keywords

  • 2-3- dipalmitoylglyceric acid
  • Erythrocyte deformability
  • extracorporeal circulation
  • microcirculation

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Changes in RBC deformability and oxygen-delivering ability in cold blood cardioplegia. / Son, Kuk Hui; Ahn, Chi Bum; Lee, Sung Ho; Noh, Insup; Shin, Eunkyoung; So, Mijin; Kim, Hyuna; Sun, Kyung; Kim, Kwang Taik.

In: Clinical Hemorheology and Microcirculation, Vol. 48, No. 4, 07.11.2011, p. 223-229.

Research output: Contribution to journalArticle

Son, Kuk Hui ; Ahn, Chi Bum ; Lee, Sung Ho ; Noh, Insup ; Shin, Eunkyoung ; So, Mijin ; Kim, Hyuna ; Sun, Kyung ; Kim, Kwang Taik. / Changes in RBC deformability and oxygen-delivering ability in cold blood cardioplegia. In: Clinical Hemorheology and Microcirculation. 2011 ; Vol. 48, No. 4. pp. 223-229.
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AU - So, Mijin

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