Changes in the arginine methylation of organ proteins during the development of diabetes mellitus

Jong Hoon Lee, Gil-Hong Park, Young Koo Lee, Jun Hyung Park

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: In this study, we examined changes in asymmetric dimethylarginine (ADMA), dimethylarginine dimethylaminohydrolase (DDAH), nitric oxide synthesis (NOS), and the arginine methylation of organ proteins during the development of diabetes in mice. Methods: Db/db mice developed significant obesity and fasting hyperglycemia during diabetogenesis. During diabetogenesis, the expression of ADMA and nNOS was increased, while that of DDAH1 and protein-arginine methyltransferase 1 (PRMT1) was decreased. Additionally, arginine methylation in the liver and adipose tissue was altered during diabetogenesis. Results: Changes were evident at 75, 60, and 52. kDa in liver tissue and at 38 and 25. kDa in adipose tissue. Collectively, DDAH and ADMA are closely associated with the development of obesity and diabetes, and the arginine methylation levels of certain proteins were changed during diabetes development. Conclusion: Protein arginine methylation plays a role in the pathogenesis of diabetes.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalDiabetes Research and Clinical Practice
Volume94
Issue number1
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Methylation
Arginine
Diabetes Mellitus
Adipose Tissue
Proteins
Protein-Arginine N-Methyltransferases
Obesity
Liver
Hyperglycemia
Fasting
Nitric Oxide
N,N-dimethylarginine
dimethylargininase

Keywords

  • Arginine methylation
  • Asymmetric dimethylarginine
  • Diabetes mellitus
  • Dimethylarginine dimethylaminohydrolase
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine
  • Endocrinology

Cite this

Changes in the arginine methylation of organ proteins during the development of diabetes mellitus. / Lee, Jong Hoon; Park, Gil-Hong; Lee, Young Koo; Park, Jun Hyung.

In: Diabetes Research and Clinical Practice, Vol. 94, No. 1, 01.10.2011, p. 111-118.

Research output: Contribution to journalArticle

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N2 - Aim: In this study, we examined changes in asymmetric dimethylarginine (ADMA), dimethylarginine dimethylaminohydrolase (DDAH), nitric oxide synthesis (NOS), and the arginine methylation of organ proteins during the development of diabetes in mice. Methods: Db/db mice developed significant obesity and fasting hyperglycemia during diabetogenesis. During diabetogenesis, the expression of ADMA and nNOS was increased, while that of DDAH1 and protein-arginine methyltransferase 1 (PRMT1) was decreased. Additionally, arginine methylation in the liver and adipose tissue was altered during diabetogenesis. Results: Changes were evident at 75, 60, and 52. kDa in liver tissue and at 38 and 25. kDa in adipose tissue. Collectively, DDAH and ADMA are closely associated with the development of obesity and diabetes, and the arginine methylation levels of certain proteins were changed during diabetes development. Conclusion: Protein arginine methylation plays a role in the pathogenesis of diabetes.

AB - Aim: In this study, we examined changes in asymmetric dimethylarginine (ADMA), dimethylarginine dimethylaminohydrolase (DDAH), nitric oxide synthesis (NOS), and the arginine methylation of organ proteins during the development of diabetes in mice. Methods: Db/db mice developed significant obesity and fasting hyperglycemia during diabetogenesis. During diabetogenesis, the expression of ADMA and nNOS was increased, while that of DDAH1 and protein-arginine methyltransferase 1 (PRMT1) was decreased. Additionally, arginine methylation in the liver and adipose tissue was altered during diabetogenesis. Results: Changes were evident at 75, 60, and 52. kDa in liver tissue and at 38 and 25. kDa in adipose tissue. Collectively, DDAH and ADMA are closely associated with the development of obesity and diabetes, and the arginine methylation levels of certain proteins were changed during diabetes development. Conclusion: Protein arginine methylation plays a role in the pathogenesis of diabetes.

KW - Arginine methylation

KW - Asymmetric dimethylarginine

KW - Diabetes mellitus

KW - Dimethylarginine dimethylaminohydrolase

KW - Nitric oxide synthase

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