Changes in the cell size of Brevundimonas diminuta using different growth agitation rates

So Hee Lee, Sang Soo Lee, Chan Wha Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Brevundimonas diminuta (ATCC 19146) is a standard organism for validation of sterilizing-grade membrane filters. Cell size is critical for the determination of retention characteristics of 0.2 μm rated membrane filters. In this study, cell size changes of B. diminuta cultured under different physiologic states and variable agitations at 50, 100 and 200 rpm were measured by a particle size analyzer and scanning electron microscope (SEM). The smallest cells were obtained at initial stationary phase in saline lactose broth (SLB) as a shaking culture at 50 rpm. Cells grown under agitation at 50, 100 and 200 rpm showed an increase of specific growth rate (μ), about 2.9, 3.6 and 3.6 fold, respectively, compared to the non-agitated cells in SLB media. These results suggested that the cell size decreased proportionally with increase of the specific growth rate (μ) in SLB. These size changes were associated with penetration through a 0.2 μm rated cellulose acetate filter. A scale-down filtration system was developed and performed bacterial challenge test and bubble point test with cells cultured in SLB. Cells grown under agitation conditions in SLB were not retained by 0.2 μm rated membrane filter.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalPDA Journal of Pharmaceutical Science and Technology
Volume56
Issue number2
Publication statusPublished - 2002 May 2

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Lactose
Cell Size
Growth
Membranes
Particle Size
Cultured Cells
Electrons

Keywords

  • Bacterial challenge test
  • Brevundimonas diminuta
  • Cell size
  • Filter validation

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Changes in the cell size of Brevundimonas diminuta using different growth agitation rates. / Lee, So Hee; Lee, Sang Soo; Kim, Chan Wha.

In: PDA Journal of Pharmaceutical Science and Technology, Vol. 56, No. 2, 02.05.2002, p. 99-108.

Research output: Contribution to journalArticle

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