CharacGrape seed proanthocyanidin inhibits inflammatory responses in hepatic stellate cells by modulating the MAPK, Akt and NF-κB signaling pathways

Jin Woo Lee, Young Il Kim, Youngchul Kim, Minji Choi, Seoyeon Min, Yong Hoon Joo, Sung Vin Yim, Namhyun Chung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the present study, we aimed to investigate the molecular mechanisms and prophylactic effects of grape seed proanthocyanidin (GSP) on lipopolysaccharide (LPS)-stimulated human hepatic stellate cells (HSCs). Cell counting and MTT assays were used to assess cell viability in the absence or presence of GSP. Reverse transcription-quantitative PCR (RT-qPCR) was performed for several inflammation-related genes (NOD1, NOD2, TLR2, TLR4, IL-1 β, IL-6, IL-8, iNOS and COX-2). The expression of anti-inflammatory cell signaling molecules, including c-Jun N-terminal kinase (JNK), p38, extracellular signal regulated kinase (ERK), Akt, nuclear factor-κB (NF-κB), inhibitory-κBα (IκBα), iNOS and COX-2, was evaluated by western blot analysis. Finally, IL-8 levels in the culture supernatant of HSCs were measured by ELISA. Pretreatment with GSP before LPS treatment significantly suppressed the mRNA expression of pro-inflammatory cytokines such as IL-1β, IL-6 and IL-8. GSP inhibited mRNA expression of LPS-induced TLR4, NOD2 and COX-2, in addition to inhibiting the expression of iNOS. GSP also inhibited LPS-induced NF-κB activation and IκBα phosphorylation. Concomitantly, GSP dose-dependently suppressed the activation of MAP kinases (JNK, ERK and p38) and Akt in LPS-stimulated HSCs. These data suggest that GSP inhibits inflammatory responses in HSCs by inactivating the NF-κB signaling pathway via MAP kinases. Thus, GSP may be considered as a novel drug for the treatment of hepatic inflammation, infectious diseases and fibrosis.

Original languageEnglish
Pages (from-to)226-234
Number of pages9
JournalInternational Journal of Molecular Medicine
Volume40
Issue number1
DOIs
Publication statusPublished - 2017 Jul 1

Fingerprint

Hepatic Stellate Cells
Seeds
Lipopolysaccharides
Interleukin-8
Extracellular Signal-Regulated MAP Kinases
Interleukin-1
Interleukin-6
Phosphotransferases
Inflammation
Messenger RNA
proanthocyanidin
Grape Seed Proanthocyanidins
MAP Kinase Signaling System
JNK Mitogen-Activated Protein Kinases
Reverse Transcription
Communicable Diseases
Cell Survival
Fibrosis
Anti-Inflammatory Agents
Western Blotting

Keywords

  • Cyclooxygenase-2
  • Grape seed proanthocyanidin
  • Hepatic stellate cells
  • Inducible nitric oxide synthase
  • Mitogen-activated protein kinase
  • Toll-like receptors

ASJC Scopus subject areas

  • Genetics

Cite this

CharacGrape seed proanthocyanidin inhibits inflammatory responses in hepatic stellate cells by modulating the MAPK, Akt and NF-κB signaling pathways. / Lee, Jin Woo; Kim, Young Il; Kim, Youngchul; Choi, Minji; Min, Seoyeon; Joo, Yong Hoon; Yim, Sung Vin; Chung, Namhyun.

In: International Journal of Molecular Medicine, Vol. 40, No. 1, 01.07.2017, p. 226-234.

Research output: Contribution to journalArticle

Lee, Jin Woo ; Kim, Young Il ; Kim, Youngchul ; Choi, Minji ; Min, Seoyeon ; Joo, Yong Hoon ; Yim, Sung Vin ; Chung, Namhyun. / CharacGrape seed proanthocyanidin inhibits inflammatory responses in hepatic stellate cells by modulating the MAPK, Akt and NF-κB signaling pathways. In: International Journal of Molecular Medicine. 2017 ; Vol. 40, No. 1. pp. 226-234.
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abstract = "In the present study, we aimed to investigate the molecular mechanisms and prophylactic effects of grape seed proanthocyanidin (GSP) on lipopolysaccharide (LPS)-stimulated human hepatic stellate cells (HSCs). Cell counting and MTT assays were used to assess cell viability in the absence or presence of GSP. Reverse transcription-quantitative PCR (RT-qPCR) was performed for several inflammation-related genes (NOD1, NOD2, TLR2, TLR4, IL-1 β, IL-6, IL-8, iNOS and COX-2). The expression of anti-inflammatory cell signaling molecules, including c-Jun N-terminal kinase (JNK), p38, extracellular signal regulated kinase (ERK), Akt, nuclear factor-κB (NF-κB), inhibitory-κBα (IκBα), iNOS and COX-2, was evaluated by western blot analysis. Finally, IL-8 levels in the culture supernatant of HSCs were measured by ELISA. Pretreatment with GSP before LPS treatment significantly suppressed the mRNA expression of pro-inflammatory cytokines such as IL-1β, IL-6 and IL-8. GSP inhibited mRNA expression of LPS-induced TLR4, NOD2 and COX-2, in addition to inhibiting the expression of iNOS. GSP also inhibited LPS-induced NF-κB activation and IκBα phosphorylation. Concomitantly, GSP dose-dependently suppressed the activation of MAP kinases (JNK, ERK and p38) and Akt in LPS-stimulated HSCs. These data suggest that GSP inhibits inflammatory responses in HSCs by inactivating the NF-κB signaling pathway via MAP kinases. Thus, GSP may be considered as a novel drug for the treatment of hepatic inflammation, infectious diseases and fibrosis.",
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AU - Lee, Jin Woo

AU - Kim, Young Il

AU - Kim, Youngchul

AU - Choi, Minji

AU - Min, Seoyeon

AU - Joo, Yong Hoon

AU - Yim, Sung Vin

AU - Chung, Namhyun

PY - 2017/7/1

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KW - Inducible nitric oxide synthase

KW - Mitogen-activated protein kinase

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