Characteristics of Epstein-Barr virus isolated from the malignant lymphomas in Korea

Insun Kim, Eui Ryun Park, Seol Hee Park, Zhenhua Lin, Young Sik Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is a common herpes virus linked to a variety of human neoplasms. In this study, the EBV detection was identified with the paraffin-embedded tissues from 62 non-Hodgkin's lymphomas, 20 Hodgkin's lymphomas, and 48 non-neoplastic tonsils, using PCR for EBNA-1 and EBER-1 mRNA in situ hybridization for EBER-1 mRNA. The isolates were analyzed for type 1/2, variants C/D and F/f, and LMP-1 30 bp deletion. EBV was isolated in 31 of 48 (66%) non-neoplastic tonsils, 24 of 42 (57%) B cell lymphomas, in 15 of 20 (75%) T cell lymphomas, and 17 of 20 (85%) Hodgkin's lymphomas. These viruses were classified as type 1 for 81% of non-neoplastic tonsils, 95% of B cell lymphomas, 93% of T cell lymphomas, and 73% of Hodgkin's diseases. Both type 1 and 2 viruses were isolated in one non-neoplastic tonsil and 3 Hodgkin's diseases. Type C virus was predominant in non-neoplastic tonsils (77%) and B cell lymphomas (75%), while type D virus was common in T cell lymphomas (71%) and Hodgkin's diseases (73%) (P<0.05). Majority of the viruses detected in nonneoplastic tonsils (93%) and malignant lymphomas (91%) were "F" prototype. LMP-1 30 bp deletion was found in high frequency in both non-neoplastic tonsils (92%) and malignant lymphomas (86%). In conclusion, most of EBV found in Korea was type 1, and "DF" genotype was more frequent in T cell lymphomas and Hodgkin's diseases than in non-neoplastic tonsils and B cell lymphomas. LMP-1 30 bp deletion did not seem to be associated with malignant lymphomas.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalJournal of Medical Virology
Volume67
Issue number1
DOIs
Publication statusPublished - 2002 Apr 9

Fingerprint

Palatine Tonsil
Korea
Human Herpesvirus 4
Lymphoma
Hodgkin Disease
T-Cell Lymphoma
B-Cell Lymphoma
Viruses
Non-Hodgkin's Lymphoma
Messenger RNA
Paraffin
In Situ Hybridization
Genotype
Polymerase Chain Reaction

Keywords

  • Epstein-Barr virus
  • Genotype
  • LMP-1
  • Malignant lymphoma

ASJC Scopus subject areas

  • Virology

Cite this

Characteristics of Epstein-Barr virus isolated from the malignant lymphomas in Korea. / Kim, Insun; Park, Eui Ryun; Park, Seol Hee; Lin, Zhenhua; Kim, Young Sik.

In: Journal of Medical Virology, Vol. 67, No. 1, 09.04.2002, p. 59-66.

Research output: Contribution to journalArticle

Kim, Insun ; Park, Eui Ryun ; Park, Seol Hee ; Lin, Zhenhua ; Kim, Young Sik. / Characteristics of Epstein-Barr virus isolated from the malignant lymphomas in Korea. In: Journal of Medical Virology. 2002 ; Vol. 67, No. 1. pp. 59-66.
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