Characteristics of peptide assimilation by Helicobacter pylori: Evidence for involvement of cell surface peptidase

Soon Kyu Yun, Kyung Min Choi, Chang Sub Uhm, Jeong Kyu Park, Se Young Hwang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Peptide assimilation by Helicobacter pylori was investigated using L-phenylalanyl-3-thia-phenylalanine (PSP) as a detector peptide; the release of thiophenol upon enzymatic hydrolysis of PSP was spectrophotometrically detected with the aid of 5,5′-dithiobis[2-nitrobenzoic acid] (DTNB). By adding PSP to whole-cell suspension, thiophenol was produced progressively, resembling that found in Esherichia coli or Staphylococcus aureus. Interestingly, the rate of thiophenol production by H. pylori in particular was markedly reduced when cells were pretreated with trypsin, indicating surface exhibition of peptidase. According to the competitive spectrophotometry using alanyl-peptides, H. pylori did not appear to assimilate PSP through the peptide transport system. No discernible PSP assimilation could be ascertained in H. pylori cells, unless provided with some additives necessary for peptidase activity, such as Ni2+ or Mg2+ and an appropriate concentration of potassium or ammonium salts. These observations strongly suggest that, regardless of a presumptive peptide transport system, peptide assimilation of H. plori appears to be highly dependent upon milieu conditions, due to unique peptidase exhibition on the cell surface.

Original languageEnglish
Pages (from-to)899-902
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number4
Publication statusPublished - 2005 Aug 1

Fingerprint

Helicobacter pylori
Peptide Hydrolases
Peptides
Nitrobenzoates
Dithionitrobenzoic Acid
Spectrophotometry
Phenylalanine
Ammonium Compounds
Trypsin
Staphylococcus aureus
Suspensions
Potassium
Hydrolysis
Salts
Primary Spontaneous Pneumothorax
thiophenol
peptide permease

Keywords

  • Detector peptide
  • Helicobacter pylori
  • Peptidase
  • Peptide transport

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Characteristics of peptide assimilation by Helicobacter pylori : Evidence for involvement of cell surface peptidase. / Yun, Soon Kyu; Choi, Kyung Min; Uhm, Chang Sub; Park, Jeong Kyu; Hwang, Se Young.

In: Journal of Microbiology and Biotechnology, Vol. 15, No. 4, 01.08.2005, p. 899-902.

Research output: Contribution to journalArticle

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