Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs

Ho Yeon Oh, Xun Jin, Jong Geun Kim, Myung Joo Oh, Xumin Pian, Jun Mo Kim, Moon Seok Yoon, Chae Ik Son, Youngsik Lee, Ki Chang Hong, Hyunggee Kim, Yun Jaie Choi, Kwang Youn Whang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: The pig, Sus scrofa domestica includes both the miniature and commercial domestic breed. These animals have influenced the human life and economies and have been studied throughout history. Although the miniature breeds are more recent and have increasingly been used in a variety of biomedical studies, their cell lines have rarely been established. Therefore, we sought to establish primary and immortal cell lines derived from both the miniature and domestic pig to better enable insight into possible in vivo growth differences. Results: The in vitro lifespan of primary domestic pig fibroblast (PF) and miniature pig fibroblast (MPF) cells using a standard 3T3 protocol was determined. Both of the primary PF and MPF cells were shown to have a two-step replicative senescence barrier. Primary MPF cells exhibited a relatively shorter lifespan and slower proliferation rate compared to those of primary PF cells. Beyond senescence barriers, lifespan-extended PF and MPF cells were eventually established and indicated spontaneous cellular immortalization. In contrast to the immortalized PF cells, immortal MPF cells showed a transformed phenotype and possessed more frequent chromosomal abnormalities and loss of p53 regulatory function. The lifespan of primary MPF and PF cells was extended by inactivation of the p53 function using transduction by SV40LT without any detectable senescent phenotype. Conclusion: These results suggest that p53 signaling might be a major determinant for the replicative senescence in the MPF cells that have the shorter lifespan and slower growth rate compared to PF cells in vitro.

Original languageEnglish
Article number20
JournalBMC Cell Biology
Volume8
DOIs
Publication statusPublished - 2007 Jun 1

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Sus scrofa
Swine
Fibroblasts
Cell Aging
Phenotype
Cell Line
Growth
Chromosome Aberrations

ASJC Scopus subject areas

  • Cell Biology

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Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs. / Oh, Ho Yeon; Jin, Xun; Kim, Jong Geun; Oh, Myung Joo; Pian, Xumin; Kim, Jun Mo; Yoon, Moon Seok; Son, Chae Ik; Lee, Youngsik; Hong, Ki Chang; Kim, Hyunggee; Choi, Yun Jaie; Whang, Kwang Youn.

In: BMC Cell Biology, Vol. 8, 20, 01.06.2007.

Research output: Contribution to journalArticle

Oh, Ho Yeon ; Jin, Xun ; Kim, Jong Geun ; Oh, Myung Joo ; Pian, Xumin ; Kim, Jun Mo ; Yoon, Moon Seok ; Son, Chae Ik ; Lee, Youngsik ; Hong, Ki Chang ; Kim, Hyunggee ; Choi, Yun Jaie ; Whang, Kwang Youn. / Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs. In: BMC Cell Biology. 2007 ; Vol. 8.
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