Characterization and cDNA cloning of hinnavin II, a cecropin family antibacterial peptide from the cabbage butterfly, Artogeia rapae

Sung Moon Yoe, Chang Soo Kang, Sung Sik Han, In Seok Bang

Research output: Contribution to journalArticle

18 Citations (Scopus)


Hinnavins, together with lysozymes, are the main types of antibacterial peptides/proteins previously isolated from the larval haemolymph of the cabbage butterfly, Artogeia rapae as part of the humoral immune response to a bacterial invasion. One of these antibacterial peptides, named hinnavin II, was purified and characterized after cDNA cloning. The purified hinnavin II was more active against Gram negative than against Gram positive bacteria. Hinnavin II also showed a powerful synergistic effect on the inhibition of bacterial growth with purified lysozyme. The cDNA has a total length of 186 bp with a 114 coding region. The deduced protein sequence contains 38 amino acids with a coding capacity of 4142.8 Da. The result of a multiple sequence alignment and phylogenetic analysis with Clustal W indicated that mature hinnavin II showed an approximately 78.9% amino acid sequence identity with cecropin A and originated from a group containing mostly lepidopteran cecropins.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Issue number2
Publication statusPublished - 2006 Jun 1



  • 5′-rapid amplification of cDNA ends (RACE)
  • Antibacterial peptide
  • Artogeia rapae
  • Cecropin
  • Hinnavin II
  • Hinnavin II gene
  • Insect immunity

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this