Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish

Kyeong Won Yoo, Cheol Hee Kim, Hae Chul Park, Seok Hyung Kim, Hyung Seok Kim, Sung Kook Hong, Sangtae Han, Myungchull Rhee, Tae Lin Huh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A complete zebrafish mespo cDNA encoding a protein of 131 amino acids with a bHLH domain in the C-terminal has been isolated. The bHLH domain of zebrafish Mespo is highly similar to those in the mouse, chick and Xenopus, sharing 82.4%, 80.4% and 74.5% amino acid identity, respectively. At 50% epiboly, the zebrafish mespo is first detected in the marginal zone of the blastoderm but excluding the prospective shield. Subsequently, mespo expression is intensified in the involuting mesoderm at 60% epiboly, and then restricted to the presomitic mesoderm (PSM) at 95% epiboly. At the 1-somite stage, mespo expression becomes reduced in the most rostral PSM. During segmentation, mespo expression is gradually downregulated at the most rostral segmental plate where cells are being coalesced to form somites. In spadetail mutant embryos, most of mespo-expressing cells were missing.

Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalDevelopment Genes and Evolution
Volume213
Issue number4
Publication statusPublished - 2003 May 1
Externally publishedYes

Fingerprint

Mesoderm
Zebrafish
Danio rerio
Somites
amino acid
gene
Blastoderm
segmentation
Genes
blastoderm
Amino Acids
embryo
shield
amino acids
genes
Xenopus
protein
embryo (animal)
Down-Regulation
Embryonic Structures

Keywords

  • bHLH
  • Mespo
  • Presomitic mesoderm
  • Spadetail
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Yoo, K. W., Kim, C. H., Park, H. C., Kim, S. H., Kim, H. S., Hong, S. K., ... Huh, T. L. (2003). Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish. Development Genes and Evolution, 213(4), 203-206.

Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish. / Yoo, Kyeong Won; Kim, Cheol Hee; Park, Hae Chul; Kim, Seok Hyung; Kim, Hyung Seok; Hong, Sung Kook; Han, Sangtae; Rhee, Myungchull; Huh, Tae Lin.

In: Development Genes and Evolution, Vol. 213, No. 4, 01.05.2003, p. 203-206.

Research output: Contribution to journalArticle

Yoo, KW, Kim, CH, Park, HC, Kim, SH, Kim, HS, Hong, SK, Han, S, Rhee, M & Huh, TL 2003, 'Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish', Development Genes and Evolution, vol. 213, no. 4, pp. 203-206.
Yoo, Kyeong Won ; Kim, Cheol Hee ; Park, Hae Chul ; Kim, Seok Hyung ; Kim, Hyung Seok ; Hong, Sung Kook ; Han, Sangtae ; Rhee, Myungchull ; Huh, Tae Lin. / Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish. In: Development Genes and Evolution. 2003 ; Vol. 213, No. 4. pp. 203-206.
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