Characterization and immunomodulation activity of Lactobacillus sakei L2 and L8 isolated from chicken cecum

Insuk Sim, Keun Tae Park, Young Hee Lim

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the potential of lactic acid bacteria (LAB) strains as probiotics. Two strains were isolated from healthy chicken cecum and their acid and bile tolerance, residual organic acids, antibacterial activity against pathogenic bacteria, and immunomodulation activity were measured. Identification of the isolated strains was performed using the API 50CHL system and phylogenetic analysis using 16S rDNA sequencing. The isolates were determined to be Lactobacillus sakei strains. The acid tolerance of strains L2 and L8 was high enough that 75% of the inoculum survived in pH 2 for 2 h. The bile tolerance of both strains was observed at a 1% Oxgall concentration in MRS broth. The production of organic acids (lactic acid and acetic acid) and pH changes during growth were monitored and the maximum concentrations were obtained after 48 h of incubation. Culture supernatants of the two LAB strains showed strong antibacterial activity against pathogenic bacteria. The heat-killed LAB cells also induced high levels of immune cell proliferation compared with the control, and stimulated IL-6 and TNF-α production in mouse macrophages. Therefore, L. sakei strains L2 and L8 can be considered suitable probiotic bacteria.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalMicrobiology and Biotechnology Letters
Volume44
Issue number2
DOIs
Publication statusPublished - 2016 Jun 1

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Immunomodulation
Cecum
Chickens
Bacteria
Lactic Acid
Probiotics
Acids
Ribosomal DNA
Bile Acids and Salts
Bile
Acetic Acid
Lactobacillus sakei
Interleukin-6
Hot Temperature
Macrophages
Cell Proliferation
Growth

Keywords

  • Antibacterial activity
  • Immunomodulation activity
  • Lactobacillus sakei

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Characterization and immunomodulation activity of Lactobacillus sakei L2 and L8 isolated from chicken cecum. / Sim, Insuk; Park, Keun Tae; Lim, Young Hee.

In: Microbiology and Biotechnology Letters, Vol. 44, No. 2, 01.06.2016, p. 201-207.

Research output: Contribution to journalArticle

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