Characterization and optimization of two methods in the immobilization of 12 bioluminescent strains

Robert J. Mitchell, Man Bock Gu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Twelve recombinant bioluminescent bacteria have been immobilized within the wells of a 96-well plate using two different matrices-agar and sol-gel. All 12 strains were immobilized within individual wells of the plates and the sensitivity of the strains and the stability of the responses were determined for select chemicals. Each strain was exposed to seven well-characterized chemicals over a wide range of concentrations to demonstrate their individual selectivity for specific toxicants. Although the sensitivity of the immobilized cells was generally lower than cultures grown in liquid media, they were comparable. For example, strain DPD1710, which responds to DNA damage was able to detect mitomycin C, a genotoxin, at a minimum concentration of 0.6 ppb. When immobilized, the lower limit of detection was between 1 and 10 ppb. Finally, using compounds that are known to elicit a response from each of the strains, the stability of the bioluminescent responses were measured over an extended period of 4 weeks. Although the activity of several strains decreased over time, the majority of the strains used in both immobilized systems were still responsive.

Original languageEnglish
Pages (from-to)192-199
Number of pages8
JournalBiosensors and Bioelectronics
Volume22
Issue number2
DOIs
Publication statusPublished - 2006 Aug 15

Fingerprint

Immobilized Cells
Mutagens
Mitomycin
Polymethyl Methacrylate
Immobilization
DNA Damage
Agar
Limit of Detection
Gels
Bacteria
Cell culture
Sol-gels
DNA
Cells
Liquids

Keywords

  • Agar
  • High throughput array
  • Immobilization
  • Recombinant bioluminescent bacteria
  • Sol-gel

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Characterization and optimization of two methods in the immobilization of 12 bioluminescent strains. / Mitchell, Robert J.; Gu, Man Bock.

In: Biosensors and Bioelectronics, Vol. 22, No. 2, 15.08.2006, p. 192-199.

Research output: Contribution to journalArticle

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