Characterization and purification of a bacteriocin produced by a potential probiotic culture, Lactobacillus acidophilus 30SC

S. Oh, S. H. Kim, R. W. Worobo

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Lactobacillus acidophilus 3OSC was tested for its potential as a probiotic culture. The strain exhibited good acid tolerance in an artificial gastric solution as well as bile resistance in media containing 0.3% bile acids. The strain produced a heat-stable antimicrobial compound that was shown to be proteinaceous in nature and, therefore, referred to as a bacteriocin. The bacteriocin was active over a wide pH range and inhibited a number of Gram-positive bacteria including Listeria ivanovii and pathogenic strains. The bacteriocin was purified by 50% ammonium sulfate precipitation followed by hydrophobic interaction column chromatography. The SDS-PAGE of the active fractions resulted in a single band with estimated molecular mass of 3.5 kDa. These results demonstrate the potential of L. acidophilus 30SC as a probiotic culture that can be utilized in the manufacturing of dairy foods and dietary supplements.

Original languageEnglish
Pages (from-to)2747-2752
Number of pages6
JournalJournal of Dairy Science
Issue number12
Publication statusPublished - 2000 Dec



  • Bacteriocin
  • Lactobacillus acidophilus
  • Probiotics

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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