Characterization of 4 TaGAST genes during spike development and seed germination and their response to exogenous phytohormones in common wheat

Yun Jeong Kim, Jae Yoon Kim, Jin Seok Yoon, Dae Yeon Kim, Min Jeong Hong, Yong Weon Seo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were obtained at various days after flowering. Expression of TaGAST1, 2, 3, and 4 was analyzed using RT-PCR at inflorescence development stages, in different tissues, and after phytohormones application. To identify proteins interacting with TaGAST1, yeast two-hybridization was performed and BiFC analysis was used for verification. TaGAST1 was expressed at the inflorescence stage and only expressed in seedlings under abscisic acid (ABA) treatment after phytohormone treatment. TaGAST2 and TaGAST3 showed moderate expression in the spike, vigorous transcript accumulation in the seedling, and up-regulation by exogenous GA in early germination stages. TaGAST4 was predominantly expressed in the seedling. Wheat cyclophilin A-1 (TaCypA1), identified as a TaGAST1-interacting protein, showed opposite expression pattern in the developing spike to TaGAST1. TaCypA1 transcript was slightly up-regulated by GA, slightly down-regulated by paclobutrazol, and was maintained after ABA treatment. The interaction of TaGAST1 with TaCypA1 is targeted to the plasma membrane. TaGAST1 was specifically expressed in the wheat spike and was stimulated by exogenous GA treatment. TaGAST2 and TaGAST3 expression in germinating seeds and seedlings was higher than that in the spike stage. TaGAST4 was not expressed in all developmental stages. TaGAST1 and TaCypA1 might be expressed antagonistically during wheat spike development.

Original languageEnglish
Pages (from-to)1435-1449
Number of pages15
JournalMolecular Biology Reports
Volume43
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Plant Growth Regulators
Germination
Triticum
Seeds
Seedlings
Inflorescence
Abscisic Acid
Genes
Cyclophilin A
Plant Development
Growth and Development
Proteins
Up-Regulation
Yeasts
Cell Membrane
gibberellic acid
Polymerase Chain Reaction

Keywords

  • Common wheat
  • Gibberellic acid-stimulated transcript gene
  • Spike development
  • TaCypA1
  • TaGASTs

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Characterization of 4 TaGAST genes during spike development and seed germination and their response to exogenous phytohormones in common wheat. / Kim, Yun Jeong; Kim, Jae Yoon; Yoon, Jin Seok; Kim, Dae Yeon; Hong, Min Jeong; Seo, Yong Weon.

In: Molecular Biology Reports, Vol. 43, No. 12, 01.12.2016, p. 1435-1449.

Research output: Contribution to journalArticle

Kim, Yun Jeong ; Kim, Jae Yoon ; Yoon, Jin Seok ; Kim, Dae Yeon ; Hong, Min Jeong ; Seo, Yong Weon. / Characterization of 4 TaGAST genes during spike development and seed germination and their response to exogenous phytohormones in common wheat. In: Molecular Biology Reports. 2016 ; Vol. 43, No. 12. pp. 1435-1449.
@article{2ec4a2f4ba5340598588d685b94b2a90,
title = "Characterization of 4 TaGAST genes during spike development and seed germination and their response to exogenous phytohormones in common wheat",
abstract = "Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were obtained at various days after flowering. Expression of TaGAST1, 2, 3, and 4 was analyzed using RT-PCR at inflorescence development stages, in different tissues, and after phytohormones application. To identify proteins interacting with TaGAST1, yeast two-hybridization was performed and BiFC analysis was used for verification. TaGAST1 was expressed at the inflorescence stage and only expressed in seedlings under abscisic acid (ABA) treatment after phytohormone treatment. TaGAST2 and TaGAST3 showed moderate expression in the spike, vigorous transcript accumulation in the seedling, and up-regulation by exogenous GA in early germination stages. TaGAST4 was predominantly expressed in the seedling. Wheat cyclophilin A-1 (TaCypA1), identified as a TaGAST1-interacting protein, showed opposite expression pattern in the developing spike to TaGAST1. TaCypA1 transcript was slightly up-regulated by GA, slightly down-regulated by paclobutrazol, and was maintained after ABA treatment. The interaction of TaGAST1 with TaCypA1 is targeted to the plasma membrane. TaGAST1 was specifically expressed in the wheat spike and was stimulated by exogenous GA treatment. TaGAST2 and TaGAST3 expression in germinating seeds and seedlings was higher than that in the spike stage. TaGAST4 was not expressed in all developmental stages. TaGAST1 and TaCypA1 might be expressed antagonistically during wheat spike development.",
keywords = "Common wheat, Gibberellic acid-stimulated transcript gene, Spike development, TaCypA1, TaGASTs",
author = "Kim, {Yun Jeong} and Kim, {Jae Yoon} and Yoon, {Jin Seok} and Kim, {Dae Yeon} and Hong, {Min Jeong} and Seo, {Yong Weon}",
year = "2016",
month = "12",
day = "1",
doi = "10.1007/s11033-016-4077-9",
language = "English",
volume = "43",
pages = "1435--1449",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "12",

}

TY - JOUR

T1 - Characterization of 4 TaGAST genes during spike development and seed germination and their response to exogenous phytohormones in common wheat

AU - Kim, Yun Jeong

AU - Kim, Jae Yoon

AU - Yoon, Jin Seok

AU - Kim, Dae Yeon

AU - Hong, Min Jeong

AU - Seo, Yong Weon

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were obtained at various days after flowering. Expression of TaGAST1, 2, 3, and 4 was analyzed using RT-PCR at inflorescence development stages, in different tissues, and after phytohormones application. To identify proteins interacting with TaGAST1, yeast two-hybridization was performed and BiFC analysis was used for verification. TaGAST1 was expressed at the inflorescence stage and only expressed in seedlings under abscisic acid (ABA) treatment after phytohormone treatment. TaGAST2 and TaGAST3 showed moderate expression in the spike, vigorous transcript accumulation in the seedling, and up-regulation by exogenous GA in early germination stages. TaGAST4 was predominantly expressed in the seedling. Wheat cyclophilin A-1 (TaCypA1), identified as a TaGAST1-interacting protein, showed opposite expression pattern in the developing spike to TaGAST1. TaCypA1 transcript was slightly up-regulated by GA, slightly down-regulated by paclobutrazol, and was maintained after ABA treatment. The interaction of TaGAST1 with TaCypA1 is targeted to the plasma membrane. TaGAST1 was specifically expressed in the wheat spike and was stimulated by exogenous GA treatment. TaGAST2 and TaGAST3 expression in germinating seeds and seedlings was higher than that in the spike stage. TaGAST4 was not expressed in all developmental stages. TaGAST1 and TaCypA1 might be expressed antagonistically during wheat spike development.

AB - Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were obtained at various days after flowering. Expression of TaGAST1, 2, 3, and 4 was analyzed using RT-PCR at inflorescence development stages, in different tissues, and after phytohormones application. To identify proteins interacting with TaGAST1, yeast two-hybridization was performed and BiFC analysis was used for verification. TaGAST1 was expressed at the inflorescence stage and only expressed in seedlings under abscisic acid (ABA) treatment after phytohormone treatment. TaGAST2 and TaGAST3 showed moderate expression in the spike, vigorous transcript accumulation in the seedling, and up-regulation by exogenous GA in early germination stages. TaGAST4 was predominantly expressed in the seedling. Wheat cyclophilin A-1 (TaCypA1), identified as a TaGAST1-interacting protein, showed opposite expression pattern in the developing spike to TaGAST1. TaCypA1 transcript was slightly up-regulated by GA, slightly down-regulated by paclobutrazol, and was maintained after ABA treatment. The interaction of TaGAST1 with TaCypA1 is targeted to the plasma membrane. TaGAST1 was specifically expressed in the wheat spike and was stimulated by exogenous GA treatment. TaGAST2 and TaGAST3 expression in germinating seeds and seedlings was higher than that in the spike stage. TaGAST4 was not expressed in all developmental stages. TaGAST1 and TaCypA1 might be expressed antagonistically during wheat spike development.

KW - Common wheat

KW - Gibberellic acid-stimulated transcript gene

KW - Spike development

KW - TaCypA1

KW - TaGASTs

UR - http://www.scopus.com/inward/record.url?scp=84988637658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84988637658&partnerID=8YFLogxK

U2 - 10.1007/s11033-016-4077-9

DO - 10.1007/s11033-016-4077-9

M3 - Article

C2 - 27649990

AN - SCOPUS:84988637658

VL - 43

SP - 1435

EP - 1449

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 12

ER -