Characterization of a β-1,4-glucosidase from a newly isolated strain of Pholiota adiposa and its application to the hydrolysis of biomass

Sujit Sadashiv Jagtap, Saurabh Sudha Dhiman, Tae Su Kim, Jinglin Li, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The highly efficient β-1,4-glucosidase (BGL)-secreting strain, Pholiota adiposa SKU0714, was isolated and identified based on its morphological features and sequence analysis of internal transcribed spacer (ITS) rDNA. P. adiposa BGL (PaBGL), which contained a carbohydrate moiety, was purified to homogeneity from P. adiposa culture supernatants by 2-step chromatography on DEAE and Sephacryl gel filtration columns. The relative molecular weight of PaBGL was 60kDa by SDS-PAGE or 59kDa by size exclusion chromatography, indicating that the enzyme is a monomer. The pH and temperature optima for hydrolysis were 5.0 and 65°C, respectively. PaBGL showed the highest activity towards p-nitrophenyl-β-d-glucopyranoside (Vmax=4390Umgprotein-1, Km=2.23molm-3) and cellobiose (Vmax=3460Umgprotein-1, Km=5.60molm-3) ever reported. Its internal amino acid sequences showed homology with hydrolases from the glycoside hydrolase family 3 (GH3), indicating that PaBGL is a member of the GH3 family. The hydrolysis of rice straw using a commercial cellulase, Celluclast® 1.5L, resulted in a higher saccharification yield with the addition of PaBGL than with Novozyme 188. PaBGL may be a good candidate for applications that convert biomasses to biofuels and chemicals.

Original languageEnglish
Pages (from-to)181-190
Number of pages10
JournalBiomass and Bioenergy
Volume54
DOIs
Publication statusPublished - 2013 Jul 1
Externally publishedYes

Fingerprint

glucosidases
hydrolysis
Hydrolysis
Biomass
Hydrolases
Saccharification
chromatography
Size exclusion chromatography
biomass
Straw
Biofuels
Carbohydrates
Chromatography
Amino acids
Gels
Enzymes
hydrolases
Monomers
Molecular weight
homology

Keywords

  • β-Glucosidase
  • Biofuel
  • Biomass
  • Cellobiose
  • Pholiota adiposa
  • Saccharification

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

Cite this

Characterization of a β-1,4-glucosidase from a newly isolated strain of Pholiota adiposa and its application to the hydrolysis of biomass. / Jagtap, Sujit Sadashiv; Dhiman, Saurabh Sudha; Kim, Tae Su; Li, Jinglin; Kang, Yun Chan; Lee, Jung Kul.

In: Biomass and Bioenergy, Vol. 54, 01.07.2013, p. 181-190.

Research output: Contribution to journalArticle

Jagtap, Sujit Sadashiv ; Dhiman, Saurabh Sudha ; Kim, Tae Su ; Li, Jinglin ; Kang, Yun Chan ; Lee, Jung Kul. / Characterization of a β-1,4-glucosidase from a newly isolated strain of Pholiota adiposa and its application to the hydrolysis of biomass. In: Biomass and Bioenergy. 2013 ; Vol. 54. pp. 181-190.
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