Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display

Hye Lim Yang, Eun Young Cho, Kyung Hee Han, Hyun Kim, Sun Jung Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Using in silico approaches, we cloned a novel mouse gene (mbu-1) that was strictly expressed in the central nervous system. mbu-1 was first identified as an EST after carrying out digital differential display for unigene libraries from various mouse tissues. The full-length cDNA sequence was obtained by extending the ends of EST by RACE. The cDNA sequence was 2611 bp long and contained an ORF of 597 AA. A positive cis-acting region was found in the neuroblastoma × glioma hybrid, NG108-15, and in human embryonic kidney HEK293 cell lines. RT-PCR and in situ hybridization analysis showed that the mbu-1 gene was only expressed in the brain and spinal cord during the embryonic stages, and throughout all regions of the adult brain, showing higher levels in the hippocampus and hypothalamus.

Original languageEnglish
Pages (from-to)144-150
Number of pages7
JournalGene
Volume395
Issue number1-2
DOIs
Publication statusPublished - 2007 Jun 15

Fingerprint

Expressed Sequence Tags
Complementary DNA
HEK293 Cells
Brain
Neuroblastoma
Glioma
Computer Simulation
Open Reading Frames
Genes
Hypothalamus
Libraries
In Situ Hybridization
Hippocampus
Spinal Cord
Central Nervous System
Kidney
Cell Line
Polymerase Chain Reaction

Keywords

  • In silico cloning
  • In situ hybridization
  • NG108-15
  • Promoter
  • Unigene

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display. / Yang, Hye Lim; Cho, Eun Young; Han, Kyung Hee; Kim, Hyun; Kim, Sun Jung.

In: Gene, Vol. 395, No. 1-2, 15.06.2007, p. 144-150.

Research output: Contribution to journalArticle

Yang, Hye Lim ; Cho, Eun Young ; Han, Kyung Hee ; Kim, Hyun ; Kim, Sun Jung. / Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display. In: Gene. 2007 ; Vol. 395, No. 1-2. pp. 144-150.
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