Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display

Hye Lim Yang, Eun Young Cho, Kyung Hee Han, Hyun Kim, Sun Jung Kim

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4 Citations (Scopus)

Abstract

Using in silico approaches, we cloned a novel mouse gene (mbu-1) that was strictly expressed in the central nervous system. mbu-1 was first identified as an EST after carrying out digital differential display for unigene libraries from various mouse tissues. The full-length cDNA sequence was obtained by extending the ends of EST by RACE. The cDNA sequence was 2611 bp long and contained an ORF of 597 AA. A positive cis-acting region was found in the neuroblastoma × glioma hybrid, NG108-15, and in human embryonic kidney HEK293 cell lines. RT-PCR and in situ hybridization analysis showed that the mbu-1 gene was only expressed in the brain and spinal cord during the embryonic stages, and throughout all regions of the adult brain, showing higher levels in the hippocampus and hypothalamus.

Original languageEnglish
Pages (from-to)144-150
Number of pages7
JournalGene
Volume395
Issue number1-2
DOIs
Publication statusPublished - 2007 Jun 15

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Keywords

  • In silico cloning
  • In situ hybridization
  • NG108-15
  • Promoter
  • Unigene

ASJC Scopus subject areas

  • Genetics

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