Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)

Jai Hoon Eum, Sung Moon Yoe, Young R. Seo, Seok W. Kang, Sung Sik Han

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume35
Issue number5
DOIs
Publication statusPublished - 2005 May 1

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Keywords

  • Expressed sequence tags
  • Modified salivary gland
  • Nf-1 protein (Nest forming protein-1)
  • Repetitive amino acid
  • Trichoptera Hydropsyche sp.

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

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