Characterization of a novel xylanase from Armillaria gemina and its immobilization onto SiO2 nanoparticles

Saurabh Sudha Dhiman, Dayanand Kalyani, Sujit Sadashiv Jagtap, Jung Rim Haw, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Enhanced catalytic activities of different lignocellulases were obtained from Armillaria gemina under statistically optimized parameters using a jar fermenter. This strain showed maximum xylanase, endoglucanase, cellobiohydrolase, and β-glucosidase activities of 1,270, 146, 34, and 15 U mL-1, respectively. Purified A. gemina xylanase (AgXyl) has the highest catalytic efficiency (k cat/K m = 1,440 mg mL -1 s-1) ever reported for any fungal xylanase, highlighting the significance of the current study. We covalently immobilized the crude xylanase preparation onto functionalized silicon oxide nanoparticles, achieving 117 % immobilization efficiency. Further immobilization caused a shift in the optimal pH and temperature, along with a fourfold improvement in the half-life of crude AgXyl. Immobilized AgXyl gave 37.8 % higher production of xylooligosaccharides compared to free enzyme. After 17 cycles, the immobilized enzyme retained 92 % of the original activity, demonstrating its potential for the synthesis of xylooligosaccharides in industrial applications.

Original languageEnglish
Pages (from-to)1081-1091
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume97
Issue number3
DOIs
Publication statusPublished - 2013 Feb
Externally publishedYes

Keywords

  • Denaturation constant
  • Enthalpy
  • Immobilization
  • SiO
  • Xylanase
  • Xylooligosaccharide

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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