Characterization of a phage library displaying random 22mer peptides

Seung Joo Lee, Jeong Hwan Lee, Brian K. Kay, Gideon Dreyfuss, Yong Keun Park, Jeong Kook Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have characterized a phage library displaying random 22mer peptides which were produced as N-terminal fusions to the pIII coat protein of M13 filamentous phages. Among the sixty phages randomly picked from the library, 25 phages had the 22mer peptide inserts. The DNA sequence analysis of the 25 inserts showed the following results: first, each nucleotide was represented almost equally at each codon position except that there were some biases toward G bases at the first position of the codons. Secondly, the expected 47 sense codons were represented. The deduced amino acid sequences of the 25 inserts were analyzed to examine its diversity. Glycine and glutamate were the two most overrepresented residues above the expected value, whereas cysteine and threonine residues were underrepresented. The range of diversity in dipeptide sequences showed that the amino acid residues were randomly distributed along the peptide insert. Acidic, basic, polar, and nonpolar amino acid residues were represented to the extent expected at most positions of the peptide inserts. The predicted isoelectric points and hydropathy indices of the 25 peptides showed that a variety of the peptides were represented in the library. These results indicate that this phage display library could be useful in fiuding ligands for a broad spectrum of receptors by affinity screening.

Original languageEnglish
Pages (from-to)347-353
Number of pages7
JournalJournal of Microbiology
Volume35
Issue number4
Publication statusPublished - 1997 Dec 1

Fingerprint

Bacteriophages
Peptides
Codon
Libraries
Amino Acid Sequence
Bacteriophage M13
Dipeptides
Isoelectric Point
Capsid Proteins
Threonine
DNA Sequence Analysis
Glycine
Cysteine
Glutamic Acid
Nucleotides
Ligands
Amino Acids

Keywords

  • E. Coli
  • M13 bacteriophage
  • Peptide
  • Phage display library
  • pIII coat protein

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Lee, S. J., Lee, J. H., Kay, B. K., Dreyfuss, G., Park, Y. K., & Kim, J. K. (1997). Characterization of a phage library displaying random 22mer peptides. Journal of Microbiology, 35(4), 347-353.

Characterization of a phage library displaying random 22mer peptides. / Lee, Seung Joo; Lee, Jeong Hwan; Kay, Brian K.; Dreyfuss, Gideon; Park, Yong Keun; Kim, Jeong Kook.

In: Journal of Microbiology, Vol. 35, No. 4, 01.12.1997, p. 347-353.

Research output: Contribution to journalArticle

Lee, SJ, Lee, JH, Kay, BK, Dreyfuss, G, Park, YK & Kim, JK 1997, 'Characterization of a phage library displaying random 22mer peptides', Journal of Microbiology, vol. 35, no. 4, pp. 347-353.
Lee SJ, Lee JH, Kay BK, Dreyfuss G, Park YK, Kim JK. Characterization of a phage library displaying random 22mer peptides. Journal of Microbiology. 1997 Dec 1;35(4):347-353.
Lee, Seung Joo ; Lee, Jeong Hwan ; Kay, Brian K. ; Dreyfuss, Gideon ; Park, Yong Keun ; Kim, Jeong Kook. / Characterization of a phage library displaying random 22mer peptides. In: Journal of Microbiology. 1997 ; Vol. 35, No. 4. pp. 347-353.
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