Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans

Hee Taek Kim, Hyeok Jin Ko, Nahyun Kim, Duwoon Kim, Dongho Lee, In-Geol Choi, Hee Chul Woo, Myoung Dong Kim, Kyoung Heon Kim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.

Original languageEnglish
Pages (from-to)1087-1092
Number of pages6
JournalBiotechnology Letters
Volume34
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

Keywords

  • Alg7d
  • Endo-type alginate lyase
  • Polysaccharide lyase-7
  • Saccharophagus degradans

ASJC Scopus subject areas

  • Biotechnology

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