Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans

Hee Taek Kim, Hyeok Jin Ko, Nahyun Kim, Duwoon Kim, Dongho Lee, In Geol Choi, Hee Chul Woo, Myoung Dong Kim, Kyoung Heon Kim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.

Original languageEnglish
Pages (from-to)1087-1092
Number of pages6
JournalBiotechnology letters
Volume34
Issue number6
DOIs
Publication statusPublished - 2012 Jun

Keywords

  • Alg7d
  • Endo-type alginate lyase
  • Polysaccharide lyase-7
  • Saccharophagus degradans

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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