A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.
- Endo-type alginate lyase
- Polysaccharide lyase-7
- Saccharophagus degradans
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology