Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody

Jung Wook Lee, Sang Jun Sim, Sung Min Cho, Jeewon Lee

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.

Original languageEnglish
Pages (from-to)1422-1427
Number of pages6
JournalBiosensors and Bioelectronics
Volume20
Issue number7
DOIs
Publication statusPublished - 2005 Jan 15

Keywords

  • Glutamic acid decarboxylase
  • Immunosensor
  • Protein adsorption
  • Self-assembled monolayer
  • Streptavidin
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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