Characterization of a strong CCA-treated wood degrader, unknown Crustoderma species

Yong Seok Choi, Gyu Hyeok Kim, Young Woon Lim, Seong Hwan Kim, Yuji Imamura, Tsuyoshi Yoshimura, Jae-Jin Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, basidiomycete isolates that possessed a strong ability to degrade chromated copper arsenate (CCA)-treated wood were characterized. These fungal isolates, which were collected from CCA-treated pine log wastes, showed no recognizable morphological properties on culture media. Nucleotide sequence analysis of the large subunit rDNA of the isolates revealed that they were one species. Based on the high sequence similarity (>95%) and close phylogenetic relationship with several known species of Crustoderma, the fungal isolates characterized in this study were classified as a Crustoderma sp. In a wood degradation test, Crustoderma isolate KUC8611 produced a remarkably higher weight loss in CCA-treated Pinus radiata (68.7%), Pseudotsuga menziesii (39.7%), and Tsuga heterophylla (38.5%) wood than other evaluated basidiomycete species, including Crustoderma flavescens and Crustoderma corneum. In addition, extracellular enzymes for cellulose and protein degradation were detected when the isolates were cultured in chromogenic media, which supports the finding that isolate KUC8611 is a wood degrader. Furthermore, an in vitro test for metal tolerance revealed that isolate KUC8611 showed strong arsenic tolerance, but that it could not tolerate copper. Finally, isolate KUC8611 produced lower amounts of oxalic acid than copper-tolerant fungi such as Fomitopsis palustris and Antrodia vaillantii. To the best of our knowledge, this is the first study to report the degradation of CCA-treated wood by a Crustoderma species.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume95
Issue number3
DOIs
Publication statusPublished - 2009 Feb 12

Fingerprint

Basidiomycota
Copper
Tsuga
Antrodia
Coriolaceae
Pseudotsuga
Oxalic Acid
Pinus
Arsenic
Ribosomal DNA
Cellulose
Cornea
Proteolysis
Sequence Analysis
Culture Media
Weight Loss
Fungi
Metals
chromated copper arsenate
Enzymes

Keywords

  • Biodegradation
  • Brown rot fungi
  • CCA-treated wood
  • Crustoderma species
  • Metal tolerance

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Characterization of a strong CCA-treated wood degrader, unknown Crustoderma species. / Choi, Yong Seok; Kim, Gyu Hyeok; Lim, Young Woon; Kim, Seong Hwan; Imamura, Yuji; Yoshimura, Tsuyoshi; Kim, Jae-Jin.

In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, Vol. 95, No. 3, 12.02.2009, p. 285-293.

Research output: Contribution to journalArticle

Choi, Yong Seok ; Kim, Gyu Hyeok ; Lim, Young Woon ; Kim, Seong Hwan ; Imamura, Yuji ; Yoshimura, Tsuyoshi ; Kim, Jae-Jin. / Characterization of a strong CCA-treated wood degrader, unknown Crustoderma species. In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 2009 ; Vol. 95, No. 3. pp. 285-293.
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