Characterization of antithrombotic activity of lumbrokinase-immobilized polyurethane valves in the total artificial heart

Yongdoo Park, Eunsook Ryu, Hyunjeong Kim, Jaeseung Jeong, Jinhee Kim, Jaehee Shim, Sungmin Jeon, Youngho Jo, Wongon Kim, Byounggoo Min

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Thirty ng/mm2 lumbrokinase, a potent fibrinolytic enzyme, was immobilized in a Korean type total artificial heart (KORTAH) valve by photoreaction; polyallylamine was used as a photoreactive linker. Lumbrokinase-immobilized polyurethane valves were then fitted to the total artificial hearts of 3 healthy 50 kg lambs. In the control lamb, the valves were untreated; in one other, only valves on the right were treated; and in the remaining animal, only those on the left. Implants were in place for up to 3 days, and cardiac output was 5 L/min. To facilitate thrombus formation, low doses of heparin were administered. In the control lamb, thrombi was observed only in the inlet parts of the valves. In the other 2 experiments, thrombi formed in untreated control valves but not in lumbrokinase treated valves. The grade of thrombus formation in untreated valves was 1.06 ± 1.37 versus 0 ± 0 in the treated part by one-sided Student's t-test (p < 0.1). After implantation, fibrinolytic activity was only observed in treated valves by fibrin plate methods. The proteolytic activity of the treated valves was 3 times higher than that of untreated valves using the azocasein method. These data show that lumbrokinase treated polyurethane valves lead to decreased thrombus formation in vivo and that their biocompatibility is therefore greater than that of untreated valves.

Original languageEnglish
Pages (from-to)210-214
Number of pages5
JournalArtificial Organs
Volume23
Issue number2
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Lumbrokinase Fibrinolytic enzyme
  • Polyurethane heart valves
  • Total artificial heart

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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