Characterization of Arabidopsis thaliana FLAVONOL SYNTHASE 1 (FLS1) -overexpression plants in response to abiotic stress

Nguyen Hoai Nguyen; Jun Hyeok Kim; Jaeyoung Kwon; Chan Young Jeong; Wonje Lee; Dongho Lee; Suk Whan Hong; Hojoung Lee

doi:10.1016/j.plaphy.2016.03.010

Characterization of Arabidopsis thaliana FLAVONOL SYNTHASE 1 (FLS1) -overexpression plants in response to abiotic stress

Nguyen Hoai Nguyen, Jun Hyeok Kim, Jaeyoung Kwon, Chan Young Jeong, Wonje Lee, Dongho Lee, Suk Whan Hong, Hojoung Lee

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Flavonoids are an important group of secondary metabolites that are involved in plant growth and contribute to human health. Many studies have focused on the biosynthesis pathway, biochemical characters, and biological functions of flavonoids. In this report, we showed that overexpression of FLS1 (FLS1-OX) not only altered seed coat color (resulting in a light brown color), but also affected flavonoid accumulation. Whereas fls1-3 mutants accumulated higher anthocyanin levels, FLS1-OX seedlings had lower levels than those of the wild-type. Besides, shoot tissues of FLS1-OX plants exhibited lower flavonol levels than those of the wild-type. However, growth performance and abiotic stress tolerance of FLS1-OX, fls1-3, and wild-type plants were not significantly different. Taken together, FLS1 can be manipulated (i.e., silenced or overexpressed) to redirect the flavonoid biosynthetic pathway toward anthocyanin production without negative effects on plant growth and development.

Original language	English
Pages (from-to)	133-142
Number of pages	10
Journal	Plant Physiology and Biochemistry
Volume	103
DOIs	https://doi.org/10.1016/j.plaphy.2016.03.010
Publication status	Published - 2016 Jun 1

Keywords

Abiotic stress
Abscisic acid (ABA)
Anthocyanin
Arabidopsis thaliana
FLS1
Flavonol

ASJC Scopus subject areas

Physiology
Genetics
Plant Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.plaphy.2016.03.010

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title = "Characterization of Arabidopsis thaliana FLAVONOL SYNTHASE 1 (FLS1) -overexpression plants in response to abiotic stress",

abstract = "Flavonoids are an important group of secondary metabolites that are involved in plant growth and contribute to human health. Many studies have focused on the biosynthesis pathway, biochemical characters, and biological functions of flavonoids. In this report, we showed that overexpression of FLS1 (FLS1-OX) not only altered seed coat color (resulting in a light brown color), but also affected flavonoid accumulation. Whereas fls1-3 mutants accumulated higher anthocyanin levels, FLS1-OX seedlings had lower levels than those of the wild-type. Besides, shoot tissues of FLS1-OX plants exhibited lower flavonol levels than those of the wild-type. However, growth performance and abiotic stress tolerance of FLS1-OX, fls1-3, and wild-type plants were not significantly different. Taken together, FLS1 can be manipulated (i.e., silenced or overexpressed) to redirect the flavonoid biosynthetic pathway toward anthocyanin production without negative effects on plant growth and development.",

keywords = "Abiotic stress, Abscisic acid (ABA), Anthocyanin, Arabidopsis thaliana, FLS1, Flavonol",

author = "Nguyen, {Nguyen Hoai} and Kim, {Jun Hyeok} and Jaeyoung Kwon and Jeong, {Chan Young} and Wonje Lee and Dongho Lee and Hong, {Suk Whan} and Hojoung Lee",

note = "Funding Information: We would like to thank Dr. Christoph Ringli (Institute of Plant Biology, University of Z{\"u}rich) for donating the fls1-3 mutant seeds. This work was supported by a grant from the Institute of Life Science and Natural Resources, Korea University (to Hojoung Lee and Jun Hyeok Kim, 2013). Publisher Copyright: {\textcopyright} 2016 Elsevier Masson SAS.",

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doi = "10.1016/j.plaphy.2016.03.010",

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journal = "Plant Physiology and Biochemistry",

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AU - Nguyen, Nguyen Hoai

AU - Kim, Jun Hyeok

AU - Kwon, Jaeyoung

AU - Jeong, Chan Young

AU - Lee, Wonje

AU - Lee, Dongho

AU - Hong, Suk Whan

AU - Lee, Hojoung

N1 - Funding Information: We would like to thank Dr. Christoph Ringli (Institute of Plant Biology, University of Zürich) for donating the fls1-3 mutant seeds. This work was supported by a grant from the Institute of Life Science and Natural Resources, Korea University (to Hojoung Lee and Jun Hyeok Kim, 2013). Publisher Copyright: © 2016 Elsevier Masson SAS.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Flavonoids are an important group of secondary metabolites that are involved in plant growth and contribute to human health. Many studies have focused on the biosynthesis pathway, biochemical characters, and biological functions of flavonoids. In this report, we showed that overexpression of FLS1 (FLS1-OX) not only altered seed coat color (resulting in a light brown color), but also affected flavonoid accumulation. Whereas fls1-3 mutants accumulated higher anthocyanin levels, FLS1-OX seedlings had lower levels than those of the wild-type. Besides, shoot tissues of FLS1-OX plants exhibited lower flavonol levels than those of the wild-type. However, growth performance and abiotic stress tolerance of FLS1-OX, fls1-3, and wild-type plants were not significantly different. Taken together, FLS1 can be manipulated (i.e., silenced or overexpressed) to redirect the flavonoid biosynthetic pathway toward anthocyanin production without negative effects on plant growth and development.

AB - Flavonoids are an important group of secondary metabolites that are involved in plant growth and contribute to human health. Many studies have focused on the biosynthesis pathway, biochemical characters, and biological functions of flavonoids. In this report, we showed that overexpression of FLS1 (FLS1-OX) not only altered seed coat color (resulting in a light brown color), but also affected flavonoid accumulation. Whereas fls1-3 mutants accumulated higher anthocyanin levels, FLS1-OX seedlings had lower levels than those of the wild-type. Besides, shoot tissues of FLS1-OX plants exhibited lower flavonol levels than those of the wild-type. However, growth performance and abiotic stress tolerance of FLS1-OX, fls1-3, and wild-type plants were not significantly different. Taken together, FLS1 can be manipulated (i.e., silenced or overexpressed) to redirect the flavonoid biosynthetic pathway toward anthocyanin production without negative effects on plant growth and development.

KW - Abiotic stress

KW - Abscisic acid (ABA)

KW - Anthocyanin

KW - Arabidopsis thaliana

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KW - Flavonol

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Characterization of Arabidopsis thaliana FLAVONOL SYNTHASE 1 (FLS1) -overexpression plants in response to abiotic stress

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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