Characterization of ASC-2 as an antiatherogenic transcriptional coactivator of liver X receptors in macrophages

Hyang Kim Geun, Keunhee Park, Seon Yong Yeom, Jin Lee Kyung, Gukhan Kim, Je Sang Ko, Dong Kwon Rhee, Hoon Kim Young, Kyung Lee Hye, Won Kim Hae, Taeg Oh Goo, Ki Up Lee, Jae W. Lee, Seung Whan Kim

Research output: Contribution to journalArticle

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Abstract

Activating signal cointegrator-2 (ASC-2) functions as a transcriptional coactivator of many nuclear receptors and also plays important roles in the physiology of the liver and pancreas by interacting with liver X receptors (LXRs), which antagonize the development of atherosclerosis. This study was undertaken to establish the specific function of ASC-2 in macrophages and atherogenesis. Intriguingly, ASC-2 was more highly expressed in macrophages than in the liver and pancreas. To inhibit LXR-specific activity of ASC-2, we used DN2, which contains the C-terminal LXXLL motif of ASC-2 and thereby acts as an LXR-specific, dominant-negative mutant of ASC-2. In DN2-overexpressing transgenic macrophages, cellular cholesterol content was higher and cholesterol efflux lower than in control macrophages. DN2 reduced LXR ligand-dependent increases in the levels of ABCA1, ABCG1, and apolipoprotein E (apoE) transcripts as well as the activity of luciferase reporters driven by the LXR response elements (LXREs) of ABCA1, ABCG1, and apoE genes. These inhibitory effects of DN2 were reversed by overexpression of ASC-2. Chromatin immunoprecipitation analysis demonstrated that ASC-2 was recruited to the LXREs of the ABCA1, ABCG1, and apoE genes in a ligand-dependent manner and that DN2 interfered with the recruitment of ASC-2 to these LXREs. Furthermore, low-density lipoprotein receptor (LDLR)-null mice receiving bone marrow transplantation from DN2-transgenic mice showed accelerated atherogenesis when administered a high-fat diet. Taken together, these results indicate that suppression of the LXR-specific activity of ASC-2 results in both defective cholesterol metabolism in macrophages and accelerated atherogenesis, suggesting that ASC-2 is an antiatherogenic coactivator of LXRs in macrophages.

Original languageEnglish
Pages (from-to)966-974
Number of pages9
JournalMolecular Endocrinology
Volume23
Issue number7
DOIs
Publication statusPublished - 2009 Jul 1

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Macrophages
Atherosclerosis
Response Elements
Apolipoproteins E
Cholesterol
Pancreas
Nuclear Receptor Coactivators
Ligands
LDL Receptors
Chromatin Immunoprecipitation
Liver
High Fat Diet
Liver X Receptors
Luciferases
Bone Marrow Transplantation
Transgenic Mice
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Characterization of ASC-2 as an antiatherogenic transcriptional coactivator of liver X receptors in macrophages. / Geun, Hyang Kim; Park, Keunhee; Yeom, Seon Yong; Kyung, Jin Lee; Kim, Gukhan; Ko, Je Sang; Rhee, Dong Kwon; Young, Hoon Kim; Hye, Kyung Lee; Hae, Won Kim; Goo, Taeg Oh; Lee, Ki Up; Lee, Jae W.; Kim, Seung Whan.

In: Molecular Endocrinology, Vol. 23, No. 7, 01.07.2009, p. 966-974.

Research output: Contribution to journalArticle

Geun, HK, Park, K, Yeom, SY, Kyung, JL, Kim, G, Ko, JS, Rhee, DK, Young, HK, Hye, KL, Hae, WK, Goo, TO, Lee, KU, Lee, JW & Kim, SW 2009, 'Characterization of ASC-2 as an antiatherogenic transcriptional coactivator of liver X receptors in macrophages', Molecular Endocrinology, vol. 23, no. 7, pp. 966-974. https://doi.org/10.1210/me.2008-0308
Geun, Hyang Kim ; Park, Keunhee ; Yeom, Seon Yong ; Kyung, Jin Lee ; Kim, Gukhan ; Ko, Je Sang ; Rhee, Dong Kwon ; Young, Hoon Kim ; Hye, Kyung Lee ; Hae, Won Kim ; Goo, Taeg Oh ; Lee, Ki Up ; Lee, Jae W. ; Kim, Seung Whan. / Characterization of ASC-2 as an antiatherogenic transcriptional coactivator of liver X receptors in macrophages. In: Molecular Endocrinology. 2009 ; Vol. 23, No. 7. pp. 966-974.
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abstract = "Activating signal cointegrator-2 (ASC-2) functions as a transcriptional coactivator of many nuclear receptors and also plays important roles in the physiology of the liver and pancreas by interacting with liver X receptors (LXRs), which antagonize the development of atherosclerosis. This study was undertaken to establish the specific function of ASC-2 in macrophages and atherogenesis. Intriguingly, ASC-2 was more highly expressed in macrophages than in the liver and pancreas. To inhibit LXR-specific activity of ASC-2, we used DN2, which contains the C-terminal LXXLL motif of ASC-2 and thereby acts as an LXR-specific, dominant-negative mutant of ASC-2. In DN2-overexpressing transgenic macrophages, cellular cholesterol content was higher and cholesterol efflux lower than in control macrophages. DN2 reduced LXR ligand-dependent increases in the levels of ABCA1, ABCG1, and apolipoprotein E (apoE) transcripts as well as the activity of luciferase reporters driven by the LXR response elements (LXREs) of ABCA1, ABCG1, and apoE genes. These inhibitory effects of DN2 were reversed by overexpression of ASC-2. Chromatin immunoprecipitation analysis demonstrated that ASC-2 was recruited to the LXREs of the ABCA1, ABCG1, and apoE genes in a ligand-dependent manner and that DN2 interfered with the recruitment of ASC-2 to these LXREs. Furthermore, low-density lipoprotein receptor (LDLR)-null mice receiving bone marrow transplantation from DN2-transgenic mice showed accelerated atherogenesis when administered a high-fat diet. Taken together, these results indicate that suppression of the LXR-specific activity of ASC-2 results in both defective cholesterol metabolism in macrophages and accelerated atherogenesis, suggesting that ASC-2 is an antiatherogenic coactivator of LXRs in macrophages.",
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AU - Ko, Je Sang

AU - Rhee, Dong Kwon

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