Characterization of Brassica napus Flavonol Synthase Involved in Flavonol Biosynthesis in Brassica napus L.

Tien Thanh Vu, Chan Young Jeong, Hoai Nguyen Nguyen, Dongho Lee, Sang A. Lee, Ji Hye Kim, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recently, Brassica napus has become a very important crop for plant oil production. Flavonols, an uncolored flavonoid subclass, have a high antioxidative effect and are known to have antiproliferative, antiangiogenic, and neuropharmacological properties. In B. napus, some flavonoid structural genes have been identified, such as, BnF3H-1, BnCHS, and BnC4H-1. However, no studies on FLS genes in B. napus have been conducted. Thus, in this study, we cloned and characterized the function of BnFLS gene B. napus. By overexpression of the BnFLS gene, flavonol (kaempferol and quercetin) levels were recovered in the Arabidopsis atfls1-ko mutant. In addition, we found that the higher endogenous flavonol levels of BnFLS-ox in vitro shoots correlated with slightly higher ROS scavenging activities. Thus, our results indicate that the BnFLS gene encodes for a BnFLS enzyme that can be manipulated to specifically increase flavonol accumulation in oilseed plants and other species such as Arabidopsis.

Original languageEnglish
Pages (from-to)7819-7829
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number35
DOIs
Publication statusPublished - 2015 Sep 9

Fingerprint

Brassica napus
Biosynthesis
flavonols
Genes
biosynthesis
flavonoids
Arabidopsis
Flavonoids
plant fats and oils
gene overexpression
oxen
oilseed crops
genes
structural genes
kaempferol
Oilseeds
Flavonols
Plant Oils
quercetin
Scavenging

Keywords

  • anthocyanin
  • BnFLS
  • Brassica napus
  • flavonoids
  • flavonols
  • oilseed

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Characterization of Brassica napus Flavonol Synthase Involved in Flavonol Biosynthesis in Brassica napus L. / Vu, Tien Thanh; Jeong, Chan Young; Nguyen, Hoai Nguyen; Lee, Dongho; Lee, Sang A.; Kim, Ji Hye; Hong, Suk Whan; Lee, Hojoung.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 35, 09.09.2015, p. 7819-7829.

Research output: Contribution to journalArticle

Vu, Tien Thanh ; Jeong, Chan Young ; Nguyen, Hoai Nguyen ; Lee, Dongho ; Lee, Sang A. ; Kim, Ji Hye ; Hong, Suk Whan ; Lee, Hojoung. / Characterization of Brassica napus Flavonol Synthase Involved in Flavonol Biosynthesis in Brassica napus L. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 35. pp. 7819-7829.
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