Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA

Chul Joo Kang, Min Goo Lee, Young Sil Cho, Joong Won Lee, Yoon Jung Kyung, Jeong Sheop Shin, Eun Soo Kim, Jeong Kook Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A geranium (Pelargonium graveolens) chloroplast translational elongation factor EF-Tu (tufA) cDNA was isolated. The geranium tufA cDNA is 1,584 bp long with 20 bp of 5′ untranslated region (UTR) and 139 bp of 3′ UTR. It encodes 474 amino acids including a putative chloroplast transit peptide of 65 amino acids. The deduced polypeptides of the geranium tufA cDNA contains four GTP binding sequences in its N-terminal region and two chloroplast EF-Tu signature regions in the C-terminal region. The predicted molecular weight of the mature geranium chloroplast EF-Tu protein was about 45,000 and its amino acid sequence identity with the chloroplast EF-Tu proteins of tobacco, pea, Arabidopsis, rice, and soybean ranges from 85% to 91%. The geranium tufA appears to exist as a single copy gene like Arabidopsis and rice, whereas other known dicot plants have more than one copy in their nuclear genomes.

Original languageEnglish
Pages (from-to)579-583
Number of pages5
JournalMolecules and Cells
Volume10
Issue number5
Publication statusPublished - 2000 Oct 31

Fingerprint

Pelargonium
Geranium
Peptide Elongation Factor Tu
Chloroplasts
Complementary DNA
Arabidopsis
Amino Acids
5' Untranslated Regions
Peas
3' Untranslated Regions
Guanosine Triphosphate
Soybeans
Tobacco
Amino Acid Sequence
Proteins
Molecular Weight
Genome
Peptides
Genes

Keywords

  • Chloroplast
  • Elongation Factor EF-Tu
  • Geranium

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Kang, C. J., Lee, M. G., Cho, Y. S., Lee, J. W., Kyung, Y. J., Shin, J. S., ... Kim, J. K. (2000). Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA. Molecules and Cells, 10(5), 579-583.

Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA. / Kang, Chul Joo; Lee, Min Goo; Cho, Young Sil; Lee, Joong Won; Kyung, Yoon Jung; Shin, Jeong Sheop; Kim, Eun Soo; Kim, Jeong Kook.

In: Molecules and Cells, Vol. 10, No. 5, 31.10.2000, p. 579-583.

Research output: Contribution to journalArticle

Kang, CJ, Lee, MG, Cho, YS, Lee, JW, Kyung, YJ, Shin, JS, Kim, ES & Kim, JK 2000, 'Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA', Molecules and Cells, vol. 10, no. 5, pp. 579-583.
Kang CJ, Lee MG, Cho YS, Lee JW, Kyung YJ, Shin JS et al. Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA. Molecules and Cells. 2000 Oct 31;10(5):579-583.
Kang, Chul Joo ; Lee, Min Goo ; Cho, Young Sil ; Lee, Joong Won ; Kyung, Yoon Jung ; Shin, Jeong Sheop ; Kim, Eun Soo ; Kim, Jeong Kook. / Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA. In: Molecules and Cells. 2000 ; Vol. 10, No. 5. pp. 579-583.
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