Characterization of gltA: :luxCDABE fusion in Escherichia coli as a toxicity biosensor

Joo Myung Ahn, Byoung Chan Kim, Man Bock Gu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, ESJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.

Original languageEnglish
Pages (from-to)516-521
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume11
Issue number6
DOIs
Publication statusPublished - 2006 Nov 1

Keywords

  • Bioluminescence bacteria
  • Citrate synthase
  • DNA damage response

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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