Characterization of gltA: :luxCDABE fusion in Escherichia coli as a toxicity biosensor

Joo Myung Ahn, Byoung Chan Kim, Man Bock Gu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, ESJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.

Original languageEnglish
Pages (from-to)516-521
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume11
Issue number6
DOIs
Publication statusPublished - 2006 Nov 1

Fingerprint

Biosensing Techniques
Biosensors
Escherichia coli
DNA Damage
Toxicity
Fusion reactions
DNA
Photorhabdus
Nitrosoguanidines
Citrate (si)-Synthase
Nalidixic Acid
Citric Acid Cycle
Genes
Environmental Monitoring
Mitomycin
Oxidative stress
Oxidative Stress
Biomarkers
Plasmids
Polycyclic aromatic hydrocarbons

Keywords

  • Bioluminescence bacteria
  • Citrate synthase
  • DNA damage response

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

Characterization of gltA : :luxCDABE fusion in Escherichia coli as a toxicity biosensor. / Ahn, Joo Myung; Kim, Byoung Chan; Gu, Man Bock.

In: Biotechnology and Bioprocess Engineering, Vol. 11, No. 6, 01.11.2006, p. 516-521.

Research output: Contribution to journalArticle

@article{d8a7362b333048b7a9ce8dc6a51d4586,
title = "Characterization of gltA: :luxCDABE fusion in Escherichia coli as a toxicity biosensor",
abstract = "The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, ESJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.",
keywords = "Bioluminescence bacteria, Citrate synthase, DNA damage response",
author = "Ahn, {Joo Myung} and Kim, {Byoung Chan} and Gu, {Man Bock}",
year = "2006",
month = "11",
day = "1",
doi = "10.1007/BF02932076",
language = "English",
volume = "11",
pages = "516--521",
journal = "Biotechnology and Bioprocess Engineering",
issn = "1226-8372",
publisher = "Korean Society for Biotechnology and Bioengineering",
number = "6",

}

TY - JOUR

T1 - Characterization of gltA

T2 - :luxCDABE fusion in Escherichia coli as a toxicity biosensor

AU - Ahn, Joo Myung

AU - Kim, Byoung Chan

AU - Gu, Man Bock

PY - 2006/11/1

Y1 - 2006/11/1

N2 - The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, ESJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.

AB - The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, ESJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.

KW - Bioluminescence bacteria

KW - Citrate synthase

KW - DNA damage response

UR - http://www.scopus.com/inward/record.url?scp=33846054486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846054486&partnerID=8YFLogxK

U2 - 10.1007/BF02932076

DO - 10.1007/BF02932076

M3 - Article

AN - SCOPUS:33846054486

VL - 11

SP - 516

EP - 521

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

SN - 1226-8372

IS - 6

ER -