TY - JOUR
T1 - Characterization of H 2O-forming NADH oxidase from Streptococcus pyogenes and its application in l-rare sugar production
AU - Gao, Hui
AU - Tiwari, Manish Kumar
AU - Kang, Yun Chan
AU - Lee, Jung Kul
N1 - Funding Information:
This work was supported by the 21C Frontier Microbial Genomics and Applications Center Program, Ministry of Education, Science & Technology, Republic of Korea . This research was also supported by the Converging Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2011-50210 ).
PY - 2012/3/1
Y1 - 2012/3/1
N2 - A nicotinamide adenine dinucleotide (NADH) oxidase from Streptococcus pyogenes MGAS10394 (SpNox) was cloned and overexpressed in Escherichia coli BL21 (DE3). The purified SpNox enzyme had optimal pH and temperature of 7.0 and 55 °C, respectively, with a K m of 27.0 μM and a k cat/K m of 1.1 × 10 7 s -1 M -1. SpNox showed the highest activity among all known NADH oxidases, and site-directed mutagenesis and docking analysis shed light on the molecular basis of its unusually high activity. The characteristics of SpNox may prove to be useful for NAD + regeneration in the production of l-rare sugar.
AB - A nicotinamide adenine dinucleotide (NADH) oxidase from Streptococcus pyogenes MGAS10394 (SpNox) was cloned and overexpressed in Escherichia coli BL21 (DE3). The purified SpNox enzyme had optimal pH and temperature of 7.0 and 55 °C, respectively, with a K m of 27.0 μM and a k cat/K m of 1.1 × 10 7 s -1 M -1. SpNox showed the highest activity among all known NADH oxidases, and site-directed mutagenesis and docking analysis shed light on the molecular basis of its unusually high activity. The characteristics of SpNox may prove to be useful for NAD + regeneration in the production of l-rare sugar.
KW - Cofactor regeneration
KW - H O-forming NADH oxidase
KW - Streptococcus pyogenes
KW - l-Rare sugar
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U2 - 10.1016/j.bmcl.2012.01.049
DO - 10.1016/j.bmcl.2012.01.049
M3 - Article
C2 - 22326164
AN - SCOPUS:84857367018
VL - 22
SP - 1931
EP - 1935
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
SN - 0960-894X
IS - 5
ER -