Characterization of Imjin virus, a newly isolated hantavirus from the Ussuri white-toothed shrew (Crocidura lasiura)

Jin Won Song, Ji Kang Hae, Hun Gu Se, Sil Moon Sung, Shannon N. Bennett, Ki-Joon Song, Ju Baek Luck, Heung Chul Kim, Monica L. O'Guinn, Sung Tae Chong, Terry A. Klein, Richard Yanagihara

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Until recently, the single known exception to the rodent-hantavirus association was Thottapalayam virus (TPMV), a long-unclassified virus isolated from the Asian house shrew (Suncus murinus). Robust gene amplification techniques have now uncovered several genetically distinct hantaviruses from shrews in widely separated geographic regions. Here, we report the characterization of a newly identified hantavirus, designated Imjin virus (MJNV), isolated from the lung tissues of Ussuri white-toothed shrews of the species Crocidura lasiura (order Soricomorpha, family Soricidae, subfamily Crocidurinae) captured near the demilitarized zone in the Republic of Korea during 2004 and 2005. Seasonal trapping revealed the highest prevalence of MJNV infection during the autumn, with evidence of infected shrews' clustering in distinct foci. Also, marked male predominance among anti-MJNV immunoglobulin G antibody-positive Ussuri shrews was found, whereas the male-to-female ratio among seronegative Ussuri shrews was near 1. Plaque reduction neutralization tests showed no cross neutralization for MJNV and rodent-borne hantaviruses but one-way cross neutralization for MJNV and TPMV. The nucleotide and deduced amino acid sequences for the different MJNV genomic segments revealed nearly the same calculated distances from hantaviruses harbored by rodents in the subfamilies Murinae, Arvicolinae, Neotominae, and Sigmodontinae. Phylogenetic analyses of full-length S, M, and L segment sequences demonstrated that MJNV shared a common ancestry with TPMV and remained in a distinct out-group, suggesting early evolutionary divergence. Studies are in progress to determine if MJNV is pathogenic for humans.

Original languageEnglish
Pages (from-to)6184-6191
Number of pages8
JournalJournal of Virology
Volume83
Issue number12
DOIs
Publication statusPublished - 2009 Jun 1

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Shrews
Hantavirus
Viruses
Rodentia
Unclassified Viruses
Sigmodontinae
Murinae
Neutralization Tests
Republic of Korea
Arvicolinae
Gene Amplification
Cluster Analysis
Amino Acid Sequence
Nucleotides
Immunoglobulin G
Lung
Antibodies
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Characterization of Imjin virus, a newly isolated hantavirus from the Ussuri white-toothed shrew (Crocidura lasiura). / Song, Jin Won; Hae, Ji Kang; Se, Hun Gu; Sung, Sil Moon; Bennett, Shannon N.; Song, Ki-Joon; Luck, Ju Baek; Kim, Heung Chul; O'Guinn, Monica L.; Chong, Sung Tae; Klein, Terry A.; Yanagihara, Richard.

In: Journal of Virology, Vol. 83, No. 12, 01.06.2009, p. 6184-6191.

Research output: Contribution to journalArticle

Song, JW, Hae, JK, Se, HG, Sung, SM, Bennett, SN, Song, K-J, Luck, JB, Kim, HC, O'Guinn, ML, Chong, ST, Klein, TA & Yanagihara, R 2009, 'Characterization of Imjin virus, a newly isolated hantavirus from the Ussuri white-toothed shrew (Crocidura lasiura)', Journal of Virology, vol. 83, no. 12, pp. 6184-6191. https://doi.org/10.1128/JVI.00371-09
Song, Jin Won ; Hae, Ji Kang ; Se, Hun Gu ; Sung, Sil Moon ; Bennett, Shannon N. ; Song, Ki-Joon ; Luck, Ju Baek ; Kim, Heung Chul ; O'Guinn, Monica L. ; Chong, Sung Tae ; Klein, Terry A. ; Yanagihara, Richard. / Characterization of Imjin virus, a newly isolated hantavirus from the Ussuri white-toothed shrew (Crocidura lasiura). In: Journal of Virology. 2009 ; Vol. 83, No. 12. pp. 6184-6191.
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AU - Sung, Sil Moon

AU - Bennett, Shannon N.

AU - Song, Ki-Joon

AU - Luck, Ju Baek

AU - Kim, Heung Chul

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