Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta

Brian S. Bowser, Stephanie Morris, Moon Jung Song, Ren Sun, Blossom Damania

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


The K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters.

Original languageEnglish
Pages (from-to)309-327
Number of pages19
Issue number2
Publication statusPublished - 2006 May 10
Externally publishedYes


  • HHV8
  • K1
  • KSHV
  • Orf50
  • Rta
  • Transcription

ASJC Scopus subject areas

  • Virology


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