Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta

Brian S. Bowser, Stephanie Morris, Moon Jung Song, Ren Sun, Blossom Damania

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters.

Original languageEnglish
Pages (from-to)309-327
Number of pages19
JournalVirology
Volume348
Issue number2
DOIs
Publication statusPublished - 2006 May 10
Externally publishedYes

Fingerprint

TATA-Box Binding Protein
Human Herpesvirus 8
TATA Box
Trans-Activators
Transcription Initiation Site
DNA
Electrophoretic Mobility Shift Assay
Transcriptional Activation
Glycoproteins
B-Lymphocytes
Binding Sites
Apoptosis
Genes
Proteins

Keywords

  • HHV8
  • K1
  • KSHV
  • Orf50
  • Rta
  • Transcription

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta. / Bowser, Brian S.; Morris, Stephanie; Song, Moon Jung; Sun, Ren; Damania, Blossom.

In: Virology, Vol. 348, No. 2, 10.05.2006, p. 309-327.

Research output: Contribution to journalArticle

Bowser, Brian S. ; Morris, Stephanie ; Song, Moon Jung ; Sun, Ren ; Damania, Blossom. / Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta. In: Virology. 2006 ; Vol. 348, No. 2. pp. 309-327.
@article{7a0bfd5f34b545efacfe2ed29eb6f204,
title = "Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta",
abstract = "The K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters.",
keywords = "HHV8, K1, KSHV, Orf50, Rta, Transcription",
author = "Bowser, {Brian S.} and Stephanie Morris and Song, {Moon Jung} and Ren Sun and Blossom Damania",
year = "2006",
month = "5",
day = "10",
doi = "10.1016/j.virol.2006.02.007",
language = "English",
volume = "348",
pages = "309--327",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta

AU - Bowser, Brian S.

AU - Morris, Stephanie

AU - Song, Moon Jung

AU - Sun, Ren

AU - Damania, Blossom

PY - 2006/5/10

Y1 - 2006/5/10

N2 - The K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters.

AB - The K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters.

KW - HHV8

KW - K1

KW - KSHV

KW - Orf50

KW - Rta

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=33646486990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646486990&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2006.02.007

DO - 10.1016/j.virol.2006.02.007

M3 - Article

C2 - 16546233

AN - SCOPUS:33646486990

VL - 348

SP - 309

EP - 327

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -