Characterization of mammalian selenoprotein O: A redox-active mitochondrial protein

Seong Jeong Han, Byung Cheon Lee, Sun Hee Yim, Vadim N. Gladyshev, Seung Rock Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Selenoproteins exhibit diverse biological functions, most of which are associated with redox control. However, the functions of approximately half of mammalian selenoproteins are not known. One such protein is Selenoprotein O (SelO), the largest mammalian selenoprotein with orthologs found in a wide range of organisms, including bacteria and yeast. Here, we report characterization of mammalian SelO. Expression of this protein could be verified in HEK 293T cells by metabolic labeling of cells with 75Se, and it was abolished when selenocysteine was replaced with serine. A CxxU motif was identified in the C-terminal region of SelO. This protein was reversibly oxidized in a time- and concentration-dependent manner in HEK 293T cells when cells were treated with hydrogen peroxide. This treatment led to the formation of a transient 88 kDa SelO-containing complex. The formation of this complex was enhanced by replacing the CxxU motif with SxxC, but abolished when it was replaced with SxxS, suggesting a redox interaction of SelO with another protein through its Sec residue. SelO was localized to mitochondria and expressed across mouse tissues. Its expression was little affected by selenium deficiency, suggesting it has a high priority for selenium supply. Taken together, these results show that SelO is a redox-active mitochondrial selenoprotein.

Original languageEnglish
Article numbere95518
JournalPloS one
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 21
Externally publishedYes

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Selenoproteins
selenoproteins
Mitochondrial Proteins
Oxidation-Reduction
proteins
HEK293 Cells
Selenium
selenium
Proteins
cells
Selenocysteine
selenocysteine
Mitochondria
Yeast
serine
Labeling
Serine
Hydrogen Peroxide
hydrogen peroxide
Bacteria

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Characterization of mammalian selenoprotein O : A redox-active mitochondrial protein. / Han, Seong Jeong; Lee, Byung Cheon; Yim, Sun Hee; Gladyshev, Vadim N.; Lee, Seung Rock.

In: PloS one, Vol. 9, No. 4, e95518, 21.04.2014.

Research output: Contribution to journalArticle

Han, Seong Jeong ; Lee, Byung Cheon ; Yim, Sun Hee ; Gladyshev, Vadim N. ; Lee, Seung Rock. / Characterization of mammalian selenoprotein O : A redox-active mitochondrial protein. In: PloS one. 2014 ; Vol. 9, No. 4.
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