α-Neoagarobiose (NAB)/neoagarooligosaccharide (NAO) hydrolase plays an important role as an exo-acting 3,6-anhydro-α-(1,3)-L-galactosidase in agarose utilization. Agarose is an abun-dant polysaccharide found in red seaweeds, comprising 3,6-anhydro-L-galactose (AHG) and D-galactose residues. Unlike agarose degradation, which has been reported in marine microbes, recent metagenomic analysis of Bacteroides plebeius, a human gut bacterium, revealed the presence of genes encoding enzymes involved in agarose degradation, including α-NAB/NAO hydrolase. Among the agarolytic enzymes, BpGH117 has been partially characterized. Here, we characterized the exo-acting α-NAB/NAO hydrolase BpGH117, originating from B. plebeius. The optimal temperature and pH for His-tagged BpGH117 activity were 35◦ C and 9.0, respectively, indicative of its unique origin. His-tagged BpGH117 was thermostable up to 35◦ C, and the enzyme activity was maintained at 80% of the initial activity at a pre-incubation temperature of 40◦ C for 120 min. Km and Vmax values for NAB were 30.22 mM and 54.84 U/mg, respectively, and kcat /Km was 2.65 s−1 mM−1 . These results suggest that His-tagged BpGH117 can be used for producing bioactive products such as AHG and agarotriose from agarose efficiently.
- Bacteroides plebeius
- Exo-acting 3,6-anhydro-α-(1,3)-L-galactosidase
- Human gut bacterium
- α-neoagarooligosaccharide hydrolase
ASJC Scopus subject areas
- Drug Discovery