Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock

Ganesh D. Saratale, Min-Kyu Oh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Alkaline pretreatment using NaOH, KOH, or NaOCl has been applied to various types of waste biomass to enhance enzymatic digestibility. Pretreatment (2% NaOH, 121. °C, 30. min) of rice paddy straw (PS) resulted in a maximum yield of 703. mg of reducing sugar per gram of PS with 84.19% hydrolysis yield after a two-step enzymatic hydrolysis process. Ralstonia eutropha ATCC 17699 was tested for its ability to synthesize poly-3-hydroxybutyrate (PHB) using PS hydrolysates as its sole carbon source. It is noteworthy that dry cell weight, polyhydroxyalkanoate (PHA) accumulation and PHB yield with the use of laboratory-grade sugars were similar to those achieved with PS-derived sugars. Under optimized conditions, we observed maximal PHA accumulation (75.45%) and PHB production (11.42. g/L) within 48. h of fermentation. After PHB recovery, the physicochemical properties of PHB were determined by various analytical techniques, showed the results were consistent with the characteristics of a standard polymer of PHB. Thus, the PS hydrolysate proved to be an excellent cheap carbon substrate for PHB production.

Original languageEnglish
Pages (from-to)627-635
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume80
DOIs
Publication statusPublished - 2015 Aug 1

Fingerprint

Cupriavidus necator
Alkalies
Biomass
Feedstocks
Straw
Sugars
Polyhydroxyalkanoates
Hydrolysis
Carbon
Enzymatic hydrolysis
poly-beta-hydroxybutyrate
Fermentation
Polymers
Weights and Measures
Recovery
Substrates

Keywords

  • Alkaline pretreatment
  • Poly-3-hydroxybutyrate
  • Rice paddy straw

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Cite this

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abstract = "Alkaline pretreatment using NaOH, KOH, or NaOCl has been applied to various types of waste biomass to enhance enzymatic digestibility. Pretreatment (2{\%} NaOH, 121. °C, 30. min) of rice paddy straw (PS) resulted in a maximum yield of 703. mg of reducing sugar per gram of PS with 84.19{\%} hydrolysis yield after a two-step enzymatic hydrolysis process. Ralstonia eutropha ATCC 17699 was tested for its ability to synthesize poly-3-hydroxybutyrate (PHB) using PS hydrolysates as its sole carbon source. It is noteworthy that dry cell weight, polyhydroxyalkanoate (PHA) accumulation and PHB yield with the use of laboratory-grade sugars were similar to those achieved with PS-derived sugars. Under optimized conditions, we observed maximal PHA accumulation (75.45{\%}) and PHB production (11.42. g/L) within 48. h of fermentation. After PHB recovery, the physicochemical properties of PHB were determined by various analytical techniques, showed the results were consistent with the characteristics of a standard polymer of PHB. Thus, the PS hydrolysate proved to be an excellent cheap carbon substrate for PHB production.",
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