Characterization of proteolytically digested Zebrafish chorion as extracellular matrix

In Hong Yang, Dongho Lee, Sang Ho Lee, Ji Yoon Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Extracellular matrix (ECM) plays pivotal roles in developments, wound healing, migration, angiogenesis, and other tissue developments. Developing new ECM may give us an innovative approach of controlling the cell and tissue behavior, regenerating damaged tissues, and other biotechnology usages. Here we report new ECM like biomaterials, pFZC, obtained from the fertilized Zebrafish chorion (FZC). pFZC was prepared by digesting the fertilized Zebrafish chorion with pronase. Differentiation of P19 embryonic carcinoma cells (P19 EC) on pFZC demonstrated that the level of the differentiation of P19 EC was comparable to cells on gelatin or fibronectin coated cover slide. Analyses of glycosylates of Zebrafish chorions using various Lectins revealed that during the fertilization, structurally organized glycosylations of Zebrafish chorion were increased and FZC was glycosylated with mannose, fucose, n-acetylglucosamine, and sialic acid. In addition, after FZC proteolytic digestion with pronase, the digested FZC proteins, pFZC, displayed similar glycosylation manner. SDS gel electrophoresis and mass spectroscopy analysis of pFZC revealed that the major component of pFZC is ZP2 protein. Newly identified ECM like biomaterials, pFZC, will provide more broad ways of controlling the cell and tissue environments.

Original languageEnglish
Title of host publicationProceedings of the 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08 - "Personalized Healthcare through Technology"
Pages1837-1840
Number of pages4
Publication statusPublished - 2008 Dec 1
Event30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08 - Vancouver, BC, Canada
Duration: 2008 Aug 202008 Aug 25

Other

Other30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08
CountryCanada
CityVancouver, BC
Period08/8/2008/8/25

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ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Yang, I. H., Lee, D., Lee, S. H., & Kang, J. Y. (2008). Characterization of proteolytically digested Zebrafish chorion as extracellular matrix. In Proceedings of the 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08 - "Personalized Healthcare through Technology" (pp. 1837-1840). [4649537]