Characterization of putative cis-regulatory elements that control the transcriptional activity of the human Oct4 promoter

Heung Mo Yang, Hyun Jin Do, Jong Hyun Oh, Jin Hoi Kim, Sang Yun Choi, Kwang Yul Cha, Hyung Min Chung, Jae Hwan Kim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Octamer-binding transcription factor-4 (Oct4), a member of the POU domain transcription factors, is crucial for both early embryonic development and the maintenance of stem cell pluripotency. The human Oct4 (hOct4) 5′ upstream sequence contains four conserved regions (CR1, 2, 3, 4) that are homologous in the murine. In this study, we constructed a series of deletion mutants of the hOct4 5′ upstream region and identified cis-regulatory elements that may be important determinants for the transcriptional activity of the hOct4 promoter. Our studies showed that CR2, 3, and 4 each acted as positive cis-regulatory elements in hOct4 promoter activity. We also newly identified a putative negative cis-acting element located between CR1 and CR2. In addition, the sequence -380/-1 at CR1 that contains a GC box was sufficient to provide the minimal promoter activity. Site-directed mutagenesis and electrophoretic mobility shift assays revealed the GC box located in the -380/-1 region may play a critical role in controlling the transcriptional activity of hOct4 by the direct binding of Sp1 or Sp3 transcription factors to the GC box. An overexpression study showed that Sp1 and Sp3 positively and negatively regulate hOct4 promoter activity. Thus, the hOct4 promoter upstream region contains multiple regulatory elements, one of which, the GC box, may be an important cis-regulatory element that regulates the transcription of the hOct4 promoter by the binding of Sp family transcription factors.

Original languageEnglish
Pages (from-to)821-830
Number of pages10
JournalJournal of cellular biochemistry
Volume96
Issue number4
DOIs
Publication statusPublished - 2005 Nov 1

Keywords

  • Embryonic carcinoma cells
  • Oct4
  • Promoter
  • Sp family transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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