Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage ΦFC1

Mi Ok Park, Ki Hong Lim, Tae Hyung Kim, Hyo Ihl Chang

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Bacteriophage ΦFC1 integrase (MJ1) was previously shown to perform a site-specific recombination between a phage attachment site (attP) and a host attachment site (attB) in its host, Enterococcus faecalis, and also in a non-host bacterium, Escherichia coli. Here, we investigated biochemical features of MJ1 integrase. First, MJ1 integrase could perform in vitro recombination between attP and attB in the absence of additional factors. Second, MJ1 integrase interacted with att sites. Electrophoretic mobility shift assays and DNase I footprinting revealed that MJ1 integrase could efficiently bind to all the att sites and that MJ1 integrase recognized relatively short sequences (∼50 bp) containing an overlapping region within attB and attP. These results demonstrate that MJ1 integrase indeed catalyzes an integrative recombination between attP and attB, the mechanism of which might be simple and unidirectional, as found in serine integrases.

Original languageEnglish
Pages (from-to)342-347
Number of pages6
JournalJournal of microbiology and biotechnology
Volume17
Issue number2
Publication statusPublished - 2007 Feb

Keywords

  • Enterococcus faecalis
  • Integrase
  • attB
  • attP
  • ΦFC1

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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