Characterization of streptozotocin-induced diabetic rats and pharmacodynamics of insulin formulations

Jae Jeong Lee, Ho Young Yi, Jae Won Yang, Jun Seop Shin, Jai Hyun Kwon, Chan Wha Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Morphological and functional changes of rat pancreatic islets caused by administration of streptozotocin (STZ) and the bioavailability of insulin formulations administered to STZ-induced diabetic rats with fasting (12 h) or non-fasting were investigated. Islets isolated from normal rats maintained a good three-dimensional structure and the islet yield was 962.5 ± 86.5 islet equivalent number (IEQ, islets converted to an average diameter of 150 μm). In the diabetic group (> 500 mg/ ml blood glucose), the islet yield was only 44.4 ± 8.3 IEQ and the islet was severely damaged. The minimum reduction of blood glucose of each formulation, such as insulin solution, microcrystal, and insulin microcrystal capsule, was shown to be 11.3, 11.0, and 16.3 mg/dl, respectively, at 6 h in fasting with diabetic rats. These results indicated that the administration of insulin formulations to the fasting groups increased the severe hypoglycemic effect of insulin action more than in non-fasting diabetic rats. The diabetic rat with fasting has a regulatory disorder in maintaining the blood glucose level. Accordingly, the validity of pharmacological availability as an optimal modeling of insulin formulations is best in non-fasting STZ-induced diabetic rats.

Original languageEnglish
Pages (from-to)2396-2401
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number11
DOIs
Publication statusPublished - 2003 Nov

Keywords

  • Diabetes
  • Insulin
  • Islet
  • Pharmacodynamics
  • Streptozotocin

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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