Characterization of the full-length sequences of phospholipase A2 induced during flower development

Ji Young Kim, Young Soo Chung, Sung Han Ok, Sang Gak Lee, Won Il Chung, Ick Young Kim, Jeong Sheop Shin

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)389-392
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1489
Issue number2-3
DOIs
Publication statusPublished - 1999 Dec 23

Keywords

  • Carnation flower
  • Differential gene expression
  • Flower maturation
  • Phospholipase A
  • Suppression subtractive hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

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