Characterization of the full-length sequences of phospholipase A2 induced during flower development

Ji Young Kim, Young Soo Chung, Sung Han Ok, Sang Gak Lee, Won Il Chung, Ick Young Kim, Jeong Sheop Shin

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)389-392
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1489
Issue number2-3
DOIs
Publication statusPublished - 1999 Dec 23

Fingerprint

Dianthus
Phospholipases A2
Clone Cells
Complementary DNA
Genes
Secretory Phospholipase A2
Sequence Analysis
Animals
Amino Acids
Sequence Alignment
Northern Blotting
Amino Acid Sequence
Catalytic Domain

Keywords

  • Carnation flower
  • Differential gene expression
  • Flower maturation
  • Phospholipase A
  • Suppression subtractive hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Characterization of the full-length sequences of phospholipase A2 induced during flower development. / Kim, Ji Young; Chung, Young Soo; Ok, Sung Han; Lee, Sang Gak; Chung, Won Il; Kim, Ick Young; Shin, Jeong Sheop.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1489, No. 2-3, 23.12.1999, p. 389-392.

Research output: Contribution to journalArticle

@article{c2fe640970f04f9f8e7d78ab5195306b,
title = "Characterization of the full-length sequences of phospholipase A2 induced during flower development",
abstract = "The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.",
keywords = "Carnation flower, Differential gene expression, Flower maturation, Phospholipase A, Suppression subtractive hybridization",
author = "Kim, {Ji Young} and Chung, {Young Soo} and Ok, {Sung Han} and Lee, {Sang Gak} and Chung, {Won Il} and Kim, {Ick Young} and Shin, {Jeong Sheop}",
year = "1999",
month = "12",
day = "23",
doi = "10.1016/S0167-4781(99)00193-1",
language = "English",
volume = "1489",
pages = "389--392",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "2-3",

}

TY - JOUR

T1 - Characterization of the full-length sequences of phospholipase A2 induced during flower development

AU - Kim, Ji Young

AU - Chung, Young Soo

AU - Ok, Sung Han

AU - Lee, Sang Gak

AU - Chung, Won Il

AU - Kim, Ick Young

AU - Shin, Jeong Sheop

PY - 1999/12/23

Y1 - 1999/12/23

N2 - The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.

AB - The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.

KW - Carnation flower

KW - Differential gene expression

KW - Flower maturation

KW - Phospholipase A

KW - Suppression subtractive hybridization

UR - http://www.scopus.com/inward/record.url?scp=0032753426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032753426&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(99)00193-1

DO - 10.1016/S0167-4781(99)00193-1

M3 - Article

VL - 1489

SP - 389

EP - 392

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 2-3

ER -