Characterization of the InsP6-dependent interaction between CK2 and Nopp140

Won Kyu Lee, Sang Yeop Lee, Woo Il Kim, Yoon Hwa Rho, Young Seuk Bae, Cheolju Lee, Ick Young Kim, Yeon Gyu Yu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Nopp140, a highly phosphorylated nucleolar protein, negatively regulates CK2, a kinase essential for cell proliferation. We quantitatively analyzed the interaction between two subunits of CK2 and Nopp140 and characterized the mechanism by which InsP6 inhibits the interaction. Nopp140 specifically binds to the catalytic subunit of CK2 (CK2α) with a dissociation constant of (Kd) of 4 nM, which interferes with the catalytic activity of CK2. The C-terminal region of Nopp140 is determined as CK2α-binding region by a yeast two-hybrid method as well as a direct measurement of the interaction between CK2α and deletion mutants of Nopp140. InsP6 specifically binds to CK2α and disrupts the interaction between CK2α and Nopp140 with an IC50 value of 25 μM, thereby attenuating the Nopp140-mediated repression of CK2 activity.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume376
Issue number2
DOIs
Publication statusPublished - 2008 Nov 14

Fingerprint

Two-Hybrid System Techniques
Cell proliferation
Nuclear Proteins
Yeast
Inhibitory Concentration 50
Catalyst activity
Catalytic Domain
Phosphotransferases
Yeasts
Cell Proliferation

Keywords

  • CK2
  • InsP
  • Nopp140
  • Protein interaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Lee, W. K., Lee, S. Y., Kim, W. I., Rho, Y. H., Bae, Y. S., Lee, C., ... Yu, Y. G. (2008). Characterization of the InsP6-dependent interaction between CK2 and Nopp140. Biochemical and Biophysical Research Communications, 376(2), 439-444. https://doi.org/10.1016/j.bbrc.2008.09.008

Characterization of the InsP6-dependent interaction between CK2 and Nopp140. / Lee, Won Kyu; Lee, Sang Yeop; Kim, Woo Il; Rho, Yoon Hwa; Bae, Young Seuk; Lee, Cheolju; Kim, Ick Young; Yu, Yeon Gyu.

In: Biochemical and Biophysical Research Communications, Vol. 376, No. 2, 14.11.2008, p. 439-444.

Research output: Contribution to journalArticle

Lee, Won Kyu ; Lee, Sang Yeop ; Kim, Woo Il ; Rho, Yoon Hwa ; Bae, Young Seuk ; Lee, Cheolju ; Kim, Ick Young ; Yu, Yeon Gyu. / Characterization of the InsP6-dependent interaction between CK2 and Nopp140. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 376, No. 2. pp. 439-444.
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