Characterization of the replication region of the Enterococcus faecalis plasmid p703/5

Joon Seok Song, Jin Hwan Park, Chan Wha Kim, Young Woo Kim, Wang Jin Lim, Ick Young Kim, Hyo-Ihl Chang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this work, a 1.9-kb region of enterococcal plasmid p703/5 was isolated and the nucleotide sequence analysis of the region was performed. One major open reading frame (ORF) was identified encoding a polypeptide of 28 kDa. Database comparisons suggested that the protein showed some hornology with other bacterial RepA proteins. Upstream of the ORF, a potential dnaA box, AT-rich region and 22-bp tandemly repeated sequences (DNA iterons), a feature typical for many replication ori sites, were recognized. Deletion analysis using Exonuclease III and several restriction enzymes indicated that the three elements and the gene product from the ORF were essential for replication and that the minimum unit of DNA required for replication resided on the 1.2-kb AvaII subfragment. Thus, this gene product was referred to as RepA.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume9
Issue number1
Publication statusPublished - 1999 Feb 1

Fingerprint

Enterococcus faecalis
Open Reading Frames
Plasmids
Genes
AT Rich Sequence
DNA sequences
Polypeptides
Nucleotides
Replicon
DNA
Bacterial Proteins
Enzymes
Proteins
DNA Replication
Peptides
Sequence Analysis
Databases
exodeoxyribonuclease III

Keywords

  • AT-rich region
  • DNA iterons
  • dnaA box
  • Enterococcus faecalis
  • RepA
  • Replication ori site

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Characterization of the replication region of the Enterococcus faecalis plasmid p703/5. / Song, Joon Seok; Park, Jin Hwan; Kim, Chan Wha; Kim, Young Woo; Lim, Wang Jin; Kim, Ick Young; Chang, Hyo-Ihl.

In: Journal of Microbiology and Biotechnology, Vol. 9, No. 1, 01.02.1999, p. 91-97.

Research output: Contribution to journalArticle

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AU - Kim, Ick Young

AU - Chang, Hyo-Ihl

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AB - In this work, a 1.9-kb region of enterococcal plasmid p703/5 was isolated and the nucleotide sequence analysis of the region was performed. One major open reading frame (ORF) was identified encoding a polypeptide of 28 kDa. Database comparisons suggested that the protein showed some hornology with other bacterial RepA proteins. Upstream of the ORF, a potential dnaA box, AT-rich region and 22-bp tandemly repeated sequences (DNA iterons), a feature typical for many replication ori sites, were recognized. Deletion analysis using Exonuclease III and several restriction enzymes indicated that the three elements and the gene product from the ORF were essential for replication and that the minimum unit of DNA required for replication resided on the 1.2-kb AvaII subfragment. Thus, this gene product was referred to as RepA.

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