Characterization of the stress response of a bioluminescent biological sensor in batch and continuous cultures

Sameer P. Rupani; Man Bock Gu; Konstantin B. Konstantinov; Prasad S. Dhurjati; Tina K. Van Dyk; Robert A. LaRossa

doi:10.1021/bp960015u

Characterization of the stress response of a bioluminescent biological sensor in batch and continuous cultures

Sameer P. Rupani, Man Bock Gu, Konstantin B. Konstantinov, Prasad S. Dhurjati, Tina K. Van Dyk, Robert A. LaRossa

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

The effects of temperature, growth stage, and inducer (ethanol) concentration on the kinetics and magnitude of the stress response were investigated by using an Escherichia coli strain with the grpE heat shock promoter fused to the Vibrio fischeri lux genes. When stressed, the cells responded by changing the level of specific light emission, which was measured both on- and off-line. These measurements were used to characterize and optimize the sensitivity of the construct by determining the conditions at which the culture exhibited maximum specific bioluminescence and minimum response time to ethanol induction in batch cultivation. The results of the batch study were then applied to continuous cultivation, and the effect of dilution rate was determined. These results are of considerable interest in the development of an on-line biological sensor system for the detection and toxicity assessment of chemical pollutants.

Original language	English
Pages (from-to)	387-392
Number of pages	6
Journal	Biotechnology Progress
Volume	12
Issue number	3
DOIs	https://doi.org/10.1021/bp960015u
Publication status	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Biotechnology

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10.1021/bp960015u

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title = "Characterization of the stress response of a bioluminescent biological sensor in batch and continuous cultures",

abstract = "The effects of temperature, growth stage, and inducer (ethanol) concentration on the kinetics and magnitude of the stress response were investigated by using an Escherichia coli strain with the grpE heat shock promoter fused to the Vibrio fischeri lux genes. When stressed, the cells responded by changing the level of specific light emission, which was measured both on- and off-line. These measurements were used to characterize and optimize the sensitivity of the construct by determining the conditions at which the culture exhibited maximum specific bioluminescence and minimum response time to ethanol induction in batch cultivation. The results of the batch study were then applied to continuous cultivation, and the effect of dilution rate was determined. These results are of considerable interest in the development of an on-line biological sensor system for the detection and toxicity assessment of chemical pollutants.",

author = "Rupani, {Sameer P.} and Gu, {Man Bock} and Konstantinov, {Konstantin B.} and Dhurjati, {Prasad S.} and {Van Dyk}, {Tina K.} and LaRossa, {Robert A.}",

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doi = "10.1021/bp960015u",

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T1 - Characterization of the stress response of a bioluminescent biological sensor in batch and continuous cultures

AU - Rupani, Sameer P.

AU - Gu, Man Bock

AU - Konstantinov, Konstantin B.

AU - Dhurjati, Prasad S.

AU - Van Dyk, Tina K.

AU - LaRossa, Robert A.

PY - 1996

Y1 - 1996

N2 - The effects of temperature, growth stage, and inducer (ethanol) concentration on the kinetics and magnitude of the stress response were investigated by using an Escherichia coli strain with the grpE heat shock promoter fused to the Vibrio fischeri lux genes. When stressed, the cells responded by changing the level of specific light emission, which was measured both on- and off-line. These measurements were used to characterize and optimize the sensitivity of the construct by determining the conditions at which the culture exhibited maximum specific bioluminescence and minimum response time to ethanol induction in batch cultivation. The results of the batch study were then applied to continuous cultivation, and the effect of dilution rate was determined. These results are of considerable interest in the development of an on-line biological sensor system for the detection and toxicity assessment of chemical pollutants.

AB - The effects of temperature, growth stage, and inducer (ethanol) concentration on the kinetics and magnitude of the stress response were investigated by using an Escherichia coli strain with the grpE heat shock promoter fused to the Vibrio fischeri lux genes. When stressed, the cells responded by changing the level of specific light emission, which was measured both on- and off-line. These measurements were used to characterize and optimize the sensitivity of the construct by determining the conditions at which the culture exhibited maximum specific bioluminescence and minimum response time to ethanol induction in batch cultivation. The results of the batch study were then applied to continuous cultivation, and the effect of dilution rate was determined. These results are of considerable interest in the development of an on-line biological sensor system for the detection and toxicity assessment of chemical pollutants.

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Characterization of the stress response of a bioluminescent biological sensor in batch and continuous cultures

Abstract

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