Characterization of two frizzled8 homologues expressed in the shield and prechordal plate of zebrafish embryos

Seok Hyung Kim, Hae Chul Park, Sang Yeob Yeo, Sung Kook Hong, Jang W. Choi, Cheol H. Kim, Brant M. Weinstein, Tae L. Huh

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78%) to each other and contained an extracellular cysteine-rich domain and seven transmembrane domains that are well conserved in Fz receptor protein members. In comparison with other Fz family members, ZFz8a and ZFz8b showed the highest homology with mouse Fz8 (MFz8), sharing 84 and 76% amino acid identity, respectively. The presence of Zfz8a and Zfz8b transcripts was detected by in situ hybridization in zebrafish embryos from the 512 cell stage, and their appearance in the future dorsal region could be observed before embryos reached the 30% epiboly stage. At shield stage, Zfz8a transcripts were expressed in both epiblast and shield whereas expression of Zfz8b was only detected in the embryonic shield. During gastrula stages, both Zfz8a and Zfz8b transcripts were found in anterior dorsal regions of the involuting mesendoderm (future prechordal plate). By the 2- to 3-somite stage, expression of both Zfz8a and Zfz8b was restricted to the prechordal plate and prospective anterior neurectoderm, although expression of the Zfz8a gene was no longer present in the most anterior portion of the prechordal plate, the polster. In one-eyed pinhead mutant embryos, which lack prechordal plate, both Zfz8a and Zfz8b transcripts were reduced, confirming the prechordal plate specificity of Zfz8a and Zfz8b gene expression. These results provide an additional evidence supporting the role of Wnt signaling in organizer-mediated axial patterning.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalMechanisms of Development
Volume78
Issue number1-2
DOIs
Publication statusPublished - 1998 Dec 29
Externally publishedYes

Fingerprint

Zebrafish
Frizzled Receptors
Embryonic Structures
Gene Expression
Amino Acids
Gastrula
Germ Layers
Somites
In Situ Hybridization
Cysteine
Complementary DNA
Clone Cells
Proteins

Keywords

  • Axial mesoderm
  • Frizzled (fz)
  • Goosecoid (gsc)
  • Hypoblast
  • Neurectoderm
  • One-eyed pinhead (oep)
  • pax2
  • Polster
  • Prechordal plate
  • Pronephric duct
  • Shield
  • Wnt
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Characterization of two frizzled8 homologues expressed in the shield and prechordal plate of zebrafish embryos. / Kim, Seok Hyung; Park, Hae Chul; Yeo, Sang Yeob; Hong, Sung Kook; Choi, Jang W.; Kim, Cheol H.; Weinstein, Brant M.; Huh, Tae L.

In: Mechanisms of Development, Vol. 78, No. 1-2, 29.12.1998, p. 193-198.

Research output: Contribution to journalArticle

Kim, Seok Hyung ; Park, Hae Chul ; Yeo, Sang Yeob ; Hong, Sung Kook ; Choi, Jang W. ; Kim, Cheol H. ; Weinstein, Brant M. ; Huh, Tae L. / Characterization of two frizzled8 homologues expressed in the shield and prechordal plate of zebrafish embryos. In: Mechanisms of Development. 1998 ; Vol. 78, No. 1-2. pp. 193-198.
@article{b9743a8277bc4e299949619d9a99600c,
title = "Characterization of two frizzled8 homologues expressed in the shield and prechordal plate of zebrafish embryos",
abstract = "We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78{\%}) to each other and contained an extracellular cysteine-rich domain and seven transmembrane domains that are well conserved in Fz receptor protein members. In comparison with other Fz family members, ZFz8a and ZFz8b showed the highest homology with mouse Fz8 (MFz8), sharing 84 and 76{\%} amino acid identity, respectively. The presence of Zfz8a and Zfz8b transcripts was detected by in situ hybridization in zebrafish embryos from the 512 cell stage, and their appearance in the future dorsal region could be observed before embryos reached the 30{\%} epiboly stage. At shield stage, Zfz8a transcripts were expressed in both epiblast and shield whereas expression of Zfz8b was only detected in the embryonic shield. During gastrula stages, both Zfz8a and Zfz8b transcripts were found in anterior dorsal regions of the involuting mesendoderm (future prechordal plate). By the 2- to 3-somite stage, expression of both Zfz8a and Zfz8b was restricted to the prechordal plate and prospective anterior neurectoderm, although expression of the Zfz8a gene was no longer present in the most anterior portion of the prechordal plate, the polster. In one-eyed pinhead mutant embryos, which lack prechordal plate, both Zfz8a and Zfz8b transcripts were reduced, confirming the prechordal plate specificity of Zfz8a and Zfz8b gene expression. These results provide an additional evidence supporting the role of Wnt signaling in organizer-mediated axial patterning.",
keywords = "Axial mesoderm, Frizzled (fz), Goosecoid (gsc), Hypoblast, Neurectoderm, One-eyed pinhead (oep), pax2, Polster, Prechordal plate, Pronephric duct, Shield, Wnt, Zebrafish",
author = "Kim, {Seok Hyung} and Park, {Hae Chul} and Yeo, {Sang Yeob} and Hong, {Sung Kook} and Choi, {Jang W.} and Kim, {Cheol H.} and Weinstein, {Brant M.} and Huh, {Tae L.}",
year = "1998",
month = "12",
day = "29",
doi = "10.1016/S0925-4773(98)00137-3",
language = "English",
volume = "78",
pages = "193--198",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Characterization of two frizzled8 homologues expressed in the shield and prechordal plate of zebrafish embryos

AU - Kim, Seok Hyung

AU - Park, Hae Chul

AU - Yeo, Sang Yeob

AU - Hong, Sung Kook

AU - Choi, Jang W.

AU - Kim, Cheol H.

AU - Weinstein, Brant M.

AU - Huh, Tae L.

PY - 1998/12/29

Y1 - 1998/12/29

N2 - We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78%) to each other and contained an extracellular cysteine-rich domain and seven transmembrane domains that are well conserved in Fz receptor protein members. In comparison with other Fz family members, ZFz8a and ZFz8b showed the highest homology with mouse Fz8 (MFz8), sharing 84 and 76% amino acid identity, respectively. The presence of Zfz8a and Zfz8b transcripts was detected by in situ hybridization in zebrafish embryos from the 512 cell stage, and their appearance in the future dorsal region could be observed before embryos reached the 30% epiboly stage. At shield stage, Zfz8a transcripts were expressed in both epiblast and shield whereas expression of Zfz8b was only detected in the embryonic shield. During gastrula stages, both Zfz8a and Zfz8b transcripts were found in anterior dorsal regions of the involuting mesendoderm (future prechordal plate). By the 2- to 3-somite stage, expression of both Zfz8a and Zfz8b was restricted to the prechordal plate and prospective anterior neurectoderm, although expression of the Zfz8a gene was no longer present in the most anterior portion of the prechordal plate, the polster. In one-eyed pinhead mutant embryos, which lack prechordal plate, both Zfz8a and Zfz8b transcripts were reduced, confirming the prechordal plate specificity of Zfz8a and Zfz8b gene expression. These results provide an additional evidence supporting the role of Wnt signaling in organizer-mediated axial patterning.

AB - We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78%) to each other and contained an extracellular cysteine-rich domain and seven transmembrane domains that are well conserved in Fz receptor protein members. In comparison with other Fz family members, ZFz8a and ZFz8b showed the highest homology with mouse Fz8 (MFz8), sharing 84 and 76% amino acid identity, respectively. The presence of Zfz8a and Zfz8b transcripts was detected by in situ hybridization in zebrafish embryos from the 512 cell stage, and their appearance in the future dorsal region could be observed before embryos reached the 30% epiboly stage. At shield stage, Zfz8a transcripts were expressed in both epiblast and shield whereas expression of Zfz8b was only detected in the embryonic shield. During gastrula stages, both Zfz8a and Zfz8b transcripts were found in anterior dorsal regions of the involuting mesendoderm (future prechordal plate). By the 2- to 3-somite stage, expression of both Zfz8a and Zfz8b was restricted to the prechordal plate and prospective anterior neurectoderm, although expression of the Zfz8a gene was no longer present in the most anterior portion of the prechordal plate, the polster. In one-eyed pinhead mutant embryos, which lack prechordal plate, both Zfz8a and Zfz8b transcripts were reduced, confirming the prechordal plate specificity of Zfz8a and Zfz8b gene expression. These results provide an additional evidence supporting the role of Wnt signaling in organizer-mediated axial patterning.

KW - Axial mesoderm

KW - Frizzled (fz)

KW - Goosecoid (gsc)

KW - Hypoblast

KW - Neurectoderm

KW - One-eyed pinhead (oep)

KW - pax2

KW - Polster

KW - Prechordal plate

KW - Pronephric duct

KW - Shield

KW - Wnt

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=0031703611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031703611&partnerID=8YFLogxK

U2 - 10.1016/S0925-4773(98)00137-3

DO - 10.1016/S0925-4773(98)00137-3

M3 - Article

VL - 78

SP - 193

EP - 198

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 1-2

ER -